Redundant and distinct functions of the ABA response loci ABA-INSENSITIVE(ABI)5 and ABRE-BINDING FACTOR (ABF)3

被引:154
作者
Finkelstein, R [1 ]
Gampala, SSL
Lynch, TJ
Thomas, TL
Rock, CD
机构
[1] Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
[2] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA
[3] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA
基金
美国国家科学基金会;
关键词
ABA response element binding factor; ABA-responsive gene expression; ABI5; abscisic acid; Arabidopsis; germination;
D O I
10.1007/s11103-005-8767-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Abscisic acid-responsive gene expression is regulated by numerous transcription factors, including a subgroup of basic leucine zipper factors that bind to the conserved cis-acting sequences known as ABA-responsive elements. Although one of these factors, ABA-insensitive 5 (ABI5), was identified genetically, the paucity of genetic data for the other family members has left it unclear whether they perform unique functions or act redundantly to ABI5 or each other. To test for potential redundancy with ABI5, we identified the family members with most similar effects and interactions in transient expression systems (ABF3 and ABF1), then characterized loss-of-function lines for those loci. The abf1 and abf3 monogenic mutant lines had at most minimal effects on germination or seed-specific gene expression, but the enhanced ABA- and stress-resistance of abf3 abi5 double mutants revealed redundant action of these genes in multiple stress responses of seeds and seedlings. Although ABI5, ABF3, and ABF1 have some overlapping effects, they appear to antagonistically regulate each other's expression at specific stages. Consequently, loss of any one factor may be partially compensated by increased expression of other family members.
引用
收藏
页码:253 / 267
页数:15
相关论文
共 43 条
[1]   Genome-wide Insertional mutagenesis of Arabidopsis thaliana [J].
Alonso, JM ;
Stepanova, AN ;
Leisse, TJ ;
Kim, CJ ;
Chen, HM ;
Shinn, P ;
Stevenson, DK ;
Zimmerman, J ;
Barajas, P ;
Cheuk, R ;
Gadrinab, C ;
Heller, C ;
Jeske, A ;
Koesema, E ;
Meyers, CC ;
Parker, H ;
Prednis, L ;
Ansari, Y ;
Choy, N ;
Deen, H ;
Geralt, M ;
Hazari, N ;
Hom, E ;
Karnes, M ;
Mulholland, C ;
Ndubaku, R ;
Schmidt, I ;
Guzman, P ;
Aguilar-Henonin, L ;
Schmid, M ;
Weigel, D ;
Carter, DE ;
Marchand, T ;
Risseeuw, E ;
Brogden, D ;
Zeko, A ;
Crosby, WL ;
Berry, CC ;
Ecker, JR .
SCIENCE, 2003, 301 (5633) :653-657
[2]   The homologous ABI5 and EEL transcription factors function antagonistically to fine-tune gene expression during late embryogenesis [J].
Bensmihen, S ;
Rippa, S ;
Lambert, G ;
Jublot, D ;
Pautot, V ;
Granier, F ;
Giraudat, J ;
Parcy, F .
PLANT CELL, 2002, 14 (06) :1391-1403
[3]   The ABSCISIC ACID INSENSITIVE 3 (ABI3) gene is modulated by farnesylation and is involved in auxin signaling and lateral root development in Arabidopsis [J].
Brady, SM ;
Sarkar, SF ;
Bonetta, D ;
McCourt, P .
PLANT JOURNAL, 2003, 34 (01) :67-75
[4]   Regulation and role of the Arabidopsis Abscisic Acid-Insensitive 5 gene in abscisic acid, sugar, and stress response [J].
Brocard, IM ;
Lynch, TJ ;
Finkelstein, RR .
PLANT PHYSIOLOGY, 2002, 129 (04) :1533-1543
[5]   Regulatory networks in seeds integrating developmental, abscisic acid, sugar, and light signaling [J].
Brocard-Gifford, IM ;
Lynch, TJ ;
Finkelstein, RR .
PLANT PHYSIOLOGY, 2003, 131 (01) :78-92
[6]   Regulation of abscisic acid-induced transcription [J].
Busk, PK ;
Pagès, M .
PLANT MOLECULAR BIOLOGY, 1998, 37 (03) :425-435
[7]   Regulation of Arabidopsis thaliana Em genes:: role of ABI5 [J].
Carles, C ;
Bies-Etheve, N ;
Aspart, L ;
Léon-Kloosterziel, KM ;
Koornneef, M ;
Echeverria, M ;
Delseny, M .
PLANT JOURNAL, 2002, 30 (03) :373-383
[8]   ABFs, a family of ABA-responsive element binding factors [J].
Choi, HI ;
Hong, JH ;
Ha, JO ;
Kang, JY ;
Kim, SY .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (03) :1723-1730
[9]   GENOMIC SEQUENCING [J].
CHURCH, GM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995
[10]   A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].
DEVEREUX, J ;
HAEBERLI, P ;
SMITHIES, O .
NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395