Identification and expression of six family genes in mouse retina

被引：121

作者：

Kawakami, K ^{[1
]}

Ohto, H ^{[1
]}

Takizawa, T ^{[1
]}

Saito, T ^{[1
]}

机构：

[1] JICHI MED SCH,DEPT ANAT,MINAMI KAWACHI,TOCHIGI 32904,JAPAN

来源：

FEBS LETTERS | 1996年 / 393卷 / 2-3期

关键词：

Six gene family; cDNA cloning; retina development; homeobox protein; gel retardation assay; GST-fusion protein;

D O I：

10.1016/0014-5793(96)00899-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We identified five cDNA clones of the Six gene family which are expressed in retina, They are Six2, Six3 alpha and Six3 beta (which are derived from alternative splicing forms), Six5, and AREC3/Six4. All of these Six family genes possess extensive sequence similarity among each other in the so-homologous region (Six domain and homeodomain) but differ greatly in structure in some other regions, The amino acid sequence similarity of the so-homologous region to the previously identified AREC3/Six4 is 70.1% for Six2, 57.3% for Six3 alpha and Six3 beta, and 70.3% for Six5, The expression of these genes was observed in inner and outer nuclear layer, ganglion cell layer, and pigment epithelium of mouse retina by in situ hybridization. The so-homologous region of each Six family protein has specific DNA binding activity, Six5 and Six2 bind to the same sequence as does AREC3/Six4, while Six3 does not. These observations suggest that some of the Six family genes can regulate the same target genes.

引用

页码：259 / 263

页数：5

共 12 条

[1]

BOUCHER SA, 1995, HUM MOL GENET, V10, P1919

[2] THE DROSOPHILA SINE OCULIS LOCUS ENCODES A HOMEODOMAIN-CONTAINING PROTEIN REQUIRED FOR THE DEVELOPMENT OF THE ENTIRE VISUAL-SYSTEM [J].

CHEYETTE, BNR ;

GREEN, PJ ;

MARTIN, K ;

GARREN, H ;

HARTENSTEIN, V ;

ZIPURSKY, SL .

NEURON, 1994, 12 (05) :977-996

[3] MOLECULAR CHARACTERIZATION OF THE MURINE NEURAL RETINA LEUCINE-ZIPPER GENE, NRL [J].

FARJO, Q ;

JACKSON, AU ;

XU, JZ ;

GRYZENIA, M ;

SKOLNICK, C ;

AGARWAL, N ;

SWAROOP, A .

GENOMICS, 1993, 18 (02) :216-222

[4] IDENTIFICATION AND PURIFICATION OF A HUMAN IMMUNOGLOBULIN-ENHANCER-BINDING PROTEIN (NF-KB) THAT ACTIVATES TRANSCRIPTION FROM A HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 PROMOTER INVITRO [J].

KAWAKAMI, K ;

SCHEIDEREIT, C ;

ROEDER, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4700-4704

[5] DIFFERENT FACTORS BIND TO THE REGULATORY REGION OF THE NA+,K+-ATPASE ALPHA-1-SUBUNIT GENE DURING THE CELL-CYCLE [J].

KAWAKAMI, K ;

YANAGISAWA, K ;

WATANABE, Y ;

TOMINAGA, S ;

NAGANO, K .

FEBS LETTERS, 1993, 335 (02) :251-254

[6] Structure, function and expression of a murine homeobox protein AREC3, a homologue of Drosophila sine oculis gene product, and implication in development [J].

Kawakami, K ;

Ohto, H ;

Ikeda, K ;

Roeder, RG .

NUCLEIC ACIDS RESEARCH, 1996, 24 (02) :303-310

[7] DEVELOPMENTAL EXPRESSION OF A NOVEL MURINE HOMEOBOX GENE (CHX10) - EVIDENCE FOR ROLES IN DETERMINATION OF THE NEURORETINA AND INNER NUCLEAR LAYER [J].

LIU, ISC ;

CHEN, JD ;

PLODER, L ;

VIDGEN, D ;

VANDERKOOY, D ;

KALNINS, VI ;

MCINNES, RR .

NEURON, 1994, 13 (02) :377-393

[8]

Oliver G, 1995, DEVELOPMENT, V121, P4045

[9]

OLIVER G, 1995, DEVELOPMENT, V121, P693

[10] HOUSEKEEPING NA,K-ATPASE-ALPHA-1 SUBUNIT GENE PROMOTER IS COMPOSED OF MULTIPLE CIS ELEMENTS TO WHICH COMMON AND CELL TYPE-SPECIFIC FACTORS BIND [J].

SUZUKIYAGAWA, Y ;

KAWAKAMI, K ;

NAGANO, K .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (09) :4046-4055

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