Comet assay and early apoptosis

被引：134

作者：

Choucroun, P ^{[1
]}

Gillet, D

Dorange, G

Sawicki, B

Dewitte, JD

机构：

[1] CHU Morvan, Ctr Toxicovigilance, Lab Bioenotoxicol 13S, F-29609 Brest, France

[2] CHU Morvan, Fac Enviornm & Fonct Musculaire 13S, EA2217, F-29609 Brest, France

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2001年 / 478卷 / 1-2期

关键词：

comet assay; apoptosis; Yopro-1;

D O I：

10.1016/S0027-5107(01)00123-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The comet assay is a single cell gel electrophoresis test currently used as a qualitative and quantitative genotoxicity test. However, some of the results from this comet assay and current knowledge on apoptosis lead us to suspect the presence of some false positive results. The aim of this study was to ascertain if apoptotic cells can yield comet images that might distort the interpretation of the results. Using Jurkat cells, that hardly express Fas antigen, and apoptosis induction with anti-Fas antibody, it was possible to show that apoptosis can generate typical comet pictures as soon as the cells enter the apoptosis process. Therefore, comet images cannot be interpreted as a genotoxicity indicator when an apoptosis risk is present. Yopro-1 staining, that is also nearly immediate after apoptosis induction, can be used to balance comet assay results. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 96

页数：8

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