Dynamic control of nanofluidic channels in protein drug delivery vehicles

被引:65
作者
Angelova, A. [1 ]
Angejov, B. [2 ,3 ]
Lesieur, S. [1 ]
Mutafchieva, R. [3 ]
Ollivon, M. [1 ]
Bourgaux, C. [1 ]
Willumeit, R. [2 ]
Couvreur, P. [1 ]
机构
[1] Univ Paris Sud, CNRS, UMR 8612 Physicochim Pharmacotech Biopharm, F-92290 Chatenay Malabry, France
[2] GKSS Forschungszentrum Geesthacht GmbH, Inst Mat Res, D-21502 Geesthacht, Germany
[3] Bulgarian Acad Sci, Inst Biophys, BG-1113 Sofia, Bulgaria
关键词
self-assembly nanofluidic device; stimuli-responsive protein nanocarrier; bicontinuous cubic phase; aqueous nanochannel network;
D O I
10.1016/S1773-2247(08)50005-0
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Proteocubosomic nanocarriers are three-dimensional (3D) self-assembly periodic nanofluidic structures that have a surface topology of open nanochannels, which is apparent also in natural and self-assembled viral capsids. Here, proteins are nanoconfined in a hydrated self-assembly mixture of amphiphilic monoolein (MO) and octylglucoside (OG) forming cubosomic nanostructures. The generated periodic 3D nanochannel network architectures are investigated by means of synchrotron X-ray diffraction. The structural behavior of the ternary MO/OG/transferrin and MO/OG immunoglobulin systems as well as the binary MO/OG mixture is studied in excess aqueous environment in the temperature range from 1 to 99 degrees C. The results indicate that 5% OG molar fraction in protein-containing monoolein systems favors the formation of a swollen bicontinuous cubic liquid crystalline structure with large water channels (D-Large). Thus, it is feasible to dynamically adjust the diameters of the nanofluidic aqueous channels in the proteocubosomic carrier via the incorporation of a suitable amphiphilic additive. The obtained results may inspire the development of novel stimuli-responsive protein drug delivery nanovehicles and biocompatible self-regulating nanofluidic devices.
引用
收藏
页码:41 / 45
页数:5
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