Topology of the mitochondrial cAMP-dependent protein kinase and its substrates

被引：50

作者：

Sardanelli, AM

TechnikovaDobrova, Z

Speranza, F

Mazzocca, A

Scacco, S

Papa, S

机构：

[1] UNIV BARI, CNR, CTR STUDY MITOCHONDRIA & ENERGY METAB, BARI, ITALY

[2] ACAD SCI CZECH REPUBL, INST MICROBIOL, PRAGUE, CZECH REPUBLIC

来源：

FEBS LETTERS | 1996年 / 396卷 / 2-3期

关键词：

mitochondrion; protein phosphorylation; protein kinase; cyclic AMP; cyclic AMP-dependent protein kinase;

D O I：

10.1016/0014-5793(96)01112-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In intact bovine heart mitochondria, cAMP-dependent phosphorylation of 42, 29, 18 and 6.5 kDa proteins was inhibited by carboxyatractyloside. This shows that both mitochondrial cAMP-dependent protein kinase (mtPKA) and its protein substrates are localized at the matrix side of the inner mitochondrial membrane, Proteins of 42, 29, 18, and 6.5 kDa were also bound at the outer surface of mitochondria where they were phosphorylated by the added purified catalytic subunit of PKA. In the cytosol from bovine heart proteins of the above molecular weights were phosphorylated by the cytosolic PKA.

引用

收藏

页码：276 / 278

页数：3

相关论文

共 10 条

[1] DECLINE WITH AGE OF THE RESPIRATORY-CHAIN ACTIVITY IN HUMAN SKELETAL-MUSCLE [J].

BOFFOLI, D ;

SCACCO, SC ;

VERGARI, R ;

SOLARINO, G ;

SANTACROCE, G ;

PAPA, S .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1994, 1226 (01) :73-82

[2] CHEMICAL, IMMUNOLOGICAL, ENZYMATIC, AND GENETIC APPROACHES TO STUDYING THE ARRANGEMENT OF THE PEPTIDE-CHAIN OF THE ADP/ATP CARRIER IN THE MITOCHONDRIAL-MEMBRANE [J].

BRANDOLIN, G ;

LESAUX, A ;

TREZEGUET, V ;

LAUQUIN, GJM ;

VIGNAIS, PV .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1993, 25 (05) :459-472

[3] CAMP-DEPENDENT PROTEIN-KINASE ISOZYMES WITH PREFERENCE FOR HISTONE H2B AS SUBSTRATE IN MITOCHONDRIA OF BOVINE HEART [J].

BURGESS, JW ;

YAMADA, EW .

BIOCHEMISTRY AND CELL BIOLOGY, 1987, 65 (02) :137-143

[4] The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase [J].

Papa, S ;

Sardanelli, AM ;

Cocco, T ;

Speranza, F ;

Scacco, SC ;

TechnikovaDobrova, Z .

FEBS LETTERS, 1996, 379 (03) :299-301

[5]

Reed LJ, 1987, ENZYMES, P77

[6] Characterization of proteins phosphorylated by the cAMP-dependent protein kinase of bovine heart mitochondria [J].

Sardanelli, AM ;

TechnikovaDobrova, Z ;

Scacco, SC ;

Speranza, F ;

Papa, S .

FEBS LETTERS, 1995, 377 (03) :470-474

[7] LOCALIZATION OF CATALYTIC AND REGULATORY SUBUNITS OF CYCLIC AMP-DEPENDENT PROTEIN-KINASES IN MITOCHONDRIA FROM VARIOUS RAT-TISSUES [J].

SCHWOCH, G ;

TRINCZEK, B ;

BODE, C .

BIOCHEMICAL JOURNAL, 1990, 270 (01) :181-188

[8] PHOSPHORYLATION OF MITOCHONDRIAL PROTEINS IN BOVINE HEART - CHARACTERIZATION OF KINASES AND SUBSTRATES [J].

TECHNIKOVADOBROVA, Z ;

SARDANELLI, AM ;

PAPA, S .

FEBS LETTERS, 1993, 322 (01) :51-55

[9] CAMP-DEPENDENT PROTEIN-PHOSPHORYLATION IN MITOCHONDRIA OF BOVINE HEART [J].

TECHNIKOVADOBROVA, Z ;

SARDANELLI, AM ;

STANCA, MR ;

PAPA, S .

FEBS LETTERS, 1994, 350 (2-3) :187-191

[10]

[No title captured]