Porphyrinmaleimides: Towards thiol probes for cysteine residues in proteins

被引：29

作者：

Chen, Yihui ^{[1
]}

Parr, Timothy ^{[1
]}

Holmes, Andrea E. ^{[1
]}

Nakanishi, Koji ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

BIOCONJUGATE CHEMISTRY | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1021/bc700267f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Porphyrinmaleimides were synthesized and characterized. The thiol-containing amino acid L-Cysteine reacted with 58% yield with these porphyrins to form bioconjugate adducts. The new thiol-active reagents were labeled cytoplasmic cysteine 140 and 316 in rhodopsin (Rh), a G protein coupled receptor (GPCR).

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页码：5 / 9

页数：5

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