Utilization of mutants to analyze the interaction between Arabidopsis thaliana and its naturally root-associated Pseudomonas

被引：71

作者：

Persello-Cartieaux, F

David, P

Sarrobert, C

Thibaud, MC

Achouak, W

Robaglia, C

Nussaume, L ^{[1
]}

机构：

[1] CEA Cadarache, DSV, DEVM, Lab Metab Carbone,UMR 163,CNRS, F-13108 St Paul Durance, France

[2] CEA Cadarache, DSV, DEVM, Lab Ecol Microbienne Rhizosphere,UMR 163,CNRS, F-13108 St Paul Durance, France

来源：

PLANTA | 2001年 / 212卷 / 02期

关键词：

Arabidopsis; auxin; colonization (rhizobacterial); Pseudomonas; root (rhizobacterial colonization);

D O I：

10.1007/s004250000384

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A model system based on the Arabidopsis thaliana (L.) Heynh. Ws ecotype and its naturally colonizing Pseudomonas thivervalensis rhizobacteria was defined. Pseudomonas strains colonizing A. thaliana were found to modify the root architecture either in vivo or in vitro. A gnotobiotic system using bacteria labelled with green fluorescent protein revealed that P. thivervalensis exhibited a colonization profile similar to that of other rhizobacterial species, Mutants of A. thaliana affected in root hair development and possible hormone perception were used to analyze the plant genetic determinants of bacterial colonization. A screen for mutants insensitive to P. thivervalensis colonization yielded two mutants found to be auxin resistant. This further supports a proposed role for bacterial auxin in inducing morphological modifications of roots. This work paves the way for studying the interaction between plants and non-pathogenic rhizobacteria in a gnotobiotic system, derived from a natural association, where interactions between both partners can be genetically dissected.

引用

页码：190 / 198

页数：9

共 41 条

[1] Pseudomonas brassicacearum sp nov and Pseudomonas thivervalensis sp nov., two root-associated bacteria isolated from Brassica napus and Arabidopsis thaliana [J].