Characterizing monoclonal antibody epitopes by filtered gene fragment phage display

被引：26

作者：

Di Niro, R

Ferrara, F

Not, T

Bradbury, ARM

Chirdo, F

Marzari, R

Sblattero, D

机构：

[1] Univ Trieste, Dept Biol, I-34127 Trieste, Italy

[2] Univ Trieste, Dept Sci Reprod & Dev, I-34100 Trieste, Italy

[3] IRCCS, I-34100 Trieste, Italy

[4] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM 87545 USA

[5] Natl Univ La Plata, Fac Ciencias Exactas, Catedra Inmunol, RA-1900 La Plata, Argentina

来源：

BIOCHEMICAL JOURNAL | 2005年 / 388卷

关键词：

epitope mapping; beta-lactamase; phage display; transglutaminase;

D O I：

10.1042/BJ20041983

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able. to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.

引用

收藏

页码：889 / 894

页数：6

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