poly(beta-hydroxybutyrate-hydroxyvalerate) microspheres;
O/W emulsification with solvent evaporation;
encapsulation efficiency;
BSA release in vitro;
D O I:
10.1163/156856296X00552
中图分类号:
R318 [生物医学工程];
学科分类号:
0831 ;
摘要:
Spherical microporous matrix type microspheres composed of 330 kD P(HB-HV) (10.8% HV) 20%PCL II containing a range of %BSA loadings have been fabricated using a single emulsion technique with solvent evaporation. Microspheres were generated in high yield (>75%) and the percentage incorporation of BSA had no significant effect on microsphere size distribution, typically 6-50 mu m in diameter with a mean of 26.28 +/- 2.34 mu m, n = 25, for 20% BSA loaded microspheres. The loss of BSA both by partitioning into the aqueous continuous phase and through micropores generated during the precipitation of the fabrication polymer concomitant with solvent evaporation resulted in a low encapsulation efficiency (<15%). When the total cumulative release of BSA was expressed as a percentage of the actual total BSA incorporated, BSA release was only marginally influenced by the theoretical percentage loading suggesting that the amount and duration of BSA release in vitro was initially influenced as much by micropore number and diameter as by the extent of matrix BSA loading and detectable levels of BSA release could be monitored for up to 24 days.
机构:
MIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEYMIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEY
GURSEL, I
HASIRCI, V
论文数: 0引用数: 0
h-index: 0
机构:
MIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEYMIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEY
机构:
MIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEYMIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEY
GURSEL, I
HASIRCI, V
论文数: 0引用数: 0
h-index: 0
机构:
MIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEYMIDDLE EAST TECH UNIV,DEPT SCI BIOL,BIOTECHNOL RES UNIT,ANKARA 06531,TURKEY