Direct MS/MS analysis of proteins blotted on membranes by a matrix-assisted laser desorption/ionization-guadrupole ion trap-time-of-flight tandem mass spectrometer

被引：29

作者：

Nakanishi, T ^{[1
]}

Ohtsu, I ^{[1
]}

Furuta, M ^{[1
]}

Ando, E ^{[1
]}

Nishimura, O ^{[1
]}

机构：

[1] Shimadzu Co Ltd, Analyt & Measuring Instruments Div, Life Sci Lab, Nakagyo Ku, Kyoto 6048511, Japan

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 03期

关键词：

matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight mass spectrometry; electroblotting; piezoelectric ink-jet technology; on-membrane digestion;

D O I：

10.1021/pr0497834

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a system for the microscale identification of membrane-blotted proteins by proteolytic digestion using an instrument developed with piezoelectric chemical inkjet technology and MS/MS analyses of the resulting peptides with a matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight tandem mass spectrometer (MALDI-QIT-TOF MS). Using this system, bovine serum albumin was clearly identified at levels less than 100 fmol, and proteins from an Escherichia coli extract were also identified by an MS/MS ion search.

引用

页码：743 / 747

页数：5

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