Expression of functional GPR35 in human iNKT cells

被引：98

作者：

Fallarini, Silvia ^{[1
]}

Magliulo, Laura ^{[1
]}

Paoletti, Tiziana ^{[1
]}

de Lalla, Claudia ^{[2
]}

Lombardi, Grazia ^{[1
]}

机构：

[1] Univ Piemonte Orientale Amedeo Avogadro, Dipartimento Sci Chim Alimentari Farmaceut & Farm, I-28100 Novara, Italy

[2] Ist Sci San Raffaele, Canc Immunotherapy & Gene Therapy Program, Expt Immunol Unit, DIBIT, I-20132 Milan, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 398卷 / 03期

关键词：

GPR35; Human iNKT cells; Cytokines; Kynurenic acid; INVARIANT NKT CELLS; PROTEIN-COUPLED RECEPTOR; KILLER T-CELLS; KYNURENINE PATHWAY; IFN-GAMMA; ACID; DIFFERENTIATION; IDENTIFICATION; METABOLISM; MODULATION;

D O I：

10.1016/j.bbrc.2010.06.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to examine the expression of G protein-coupled receptor (GPR)35 in human invariant natural killer T (iNKT) cells and to determine the functional effects induced by selective activation of this receptor. RT-PCR analysis showed that both human iNKT cells and resting PBMC expressed GPR35: GPR35 protein resulted mostly localized in the plasma membrane, while it internalized in punctate intracellular structures following specific receptor activation (Western blot and immunofluorescence/confocal microscopy analysis). The specific activation of GPR35 by selective receptor agonists kynurenic acid (KYNA)] or 1,4-dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo 14,5-d]pyrimidine-7-one (zaprinast)] functionally correlated with a significant reduction in IL-4 release from alpha-galactosylceramide (alpha-GalCer)-activated human iNKT cells, and this effect resulted mediated by pertussis toxin (PTX)-sensitive Gi/o proteins. In conclusion, our results demonstrate that human iNKT cells express GPR35 functionally active in reducing IL-4 release. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：420 / 425

页数：6

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