Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae

被引：57

作者：

Beer, HD

McCarthy, JEG

Bornscheuer, UT

Schmid, RD

机构：

[1] Univ Stuttgart, Inst Tech Biochem, D-70569 Stuttgart, Germany

[2] MPI Biochem, AG Werner, D-82152 Martinsried, Germany

[3] UMIST, Dept Biochem, Manchester M60 1QD, Lancs, England

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1399卷 / 2-3期

关键词：

lipase; leader sequence; cloning; expression; sequence; (Rhizopus oryzae);

D O I：

10.1016/S0167-4781(98)00104-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A lipase from Rhizopus oryzae DSM 853 (ROL) was cloned from a chromosomal gene bank, sequenced and overexpressed in Escherichia coli. ROL and its precursors ProROL and PreProROL were purified and their pH and temperature profile was determined. In contrast to ROL, ProROL and PreProROL had considerably higher thermostability and a slightly higher pH optimum. Moreover, it could be demonstrated by in vitro experiments that the natural leader sequence of ROL is able to inhibit the folding supporting properties of the prosequence, resulting in a retardation of folding. In addition, there is strong evidence that all different lipase forms derived from Rhizopus sp. described in the literature are a result of different proteolytic processing and originate from the same gene. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：173 / 180

页数：8

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