Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: A rapid way to map helix-helix interactions in integral membrane proteins

被引：66

作者：

Mingarro, I

Whitley, P

Lemmon, MA

VonHeijne, G

机构：

[1] UNIV STOCKHOLM,DEPT BIOCHEM,S-10691 STOCKHOLM,SWEDEN

[2] NYU,MED CTR,DEPT PHARMACOL,NEW YORK,NY 10016

来源：

PROTEIN SCIENCE | 1996年 / 5卷 / 07期

关键词：

alpha-helix; helix packing; membrane protein; mutagenesis;

D O I：

10.1002/pro.5560050712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or functionally important segments in transmembrane helices.

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页码：1339 / 1341

页数：3

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