Monoclonal antibodies as tools to quantify mycelium of aquatic hyphomycetes

被引：19

作者：

Bermingham, S ^{[1
]}

Maltby, L ^{[1
]}

Dewey, FM ^{[1
]}

机构：

[1] UNIV SHEFFIELD,DEPT ANIM & PLANT SCI,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

NEW PHYTOLOGIST | 1996年 / 132卷 / 04期

关键词：

Alatospora acuminata; immunochemistry; fungi; Tetracladium marchalianum;

D O I：

10.1111/j.1469-8137.1996.tb01878.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Quantitative ELISAs have been developed to determine the biomass of two aquatic hyphomycetes in natural mixed assemblages. These assays employ a species specific rat monoclonal antibody (MAb) raised to Alatospora acuminata and a genus-specific murine MAb raised to Tetracladium marchalianum. The respective antigens are produced constitutively and their production is not affected by a range of culture conditions. The MAbs can also be used to study the spatial distribution of these fungi using immunofluorescence. Both antibodies recognize carbohydrate epitopes and belong to the immunoglobulin class IgM. The potential applications of these immunoassays are discussed.

引用

页码：593 / 601

页数：9

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