DNA prime/canarypox boost-based immunotherapy of chronic hepatitis B virus infection in a chimpanzee

被引：83

作者：

Pancholi, P

Lee, DH

Liu, QY

Tackney, C

Taylor, P

Perkus, M

Andrus, L

Brotman, B

Prince, AM

机构：

[1] New York Blood Ctr, Lindsley F Kimball Res Inst, Virol Lab, New York, NY 10021 USA

[2] New York Blood Ctr, Lindsley F Kimball Res Inst, Lab Epidemiol, New York, NY 10021 USA

[3] Virogenet, Troy, NY USA

[4] Liberian Inst Biomed Res, Vilab 2, Robertsfield, Liberia

来源：

HEPATOLOGY | 2001年 / 33卷 / 02期

关键词：

D O I：

10.1053/jhep.2001.21594

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

There are about 200 million chronic hepatitis B virus (HBV) carriers at high risk of development of cirrhosis and hepatocellular carcinoma. Termination of the carrier state may avert these risks. We have investigated immunotherapy for chronic HBV infection in a chimpanzee HBV carrier using recombinant DNA-based immunization followed by a recombinant canarypox booster. One week after the booster, HBV DNA declined greater than 400-fold and remained undetectable by the quantitative polymerase chain reaction (PCR) assay for 186 weeks. Plasma levels of hepatitis B surface antigen (HBsAg) declined for only a short time. The decline in HBV DNA correlated with a boost in gamma interferon production without a corresponding boost in cytotoxic T lymphocyte levels, and decline in the transcriptional template or covalently closed circular DNA level. Confirmation of these findings requires further studies in chimpanzees and/or in humans.

引用

页码：448 / 454

页数：7

共 24 条

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