Effects of resveratrol, a grape polyphenol, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells

被引：28

作者：

Shinohara, Yuko ^{[1
]}

Toyohira, Yumiko ^{[1
]}

Ueno, Susumu ^{[1
]}

Liu, Minhui ^{[1
]}

Tsutsui, Masato ^{[1
]}

Yanagihara, Nobuyuki ^{[1
]}

机构：

[1] Univ Occupat & Environm Hlth, Sch Med, Dept Pharmacol, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan

来源：

BIOCHEMICAL PHARMACOLOGY | 2007年 / 74卷 / 11期

基金：

日本学术振兴会;

关键词：

adrenal medulla; catecholamine secretion; catecholamine synthesis; nicotinic acetylcholine receptors; phytoestrogen; resveratrol;

D O I：

10.1016/j.bcp.2007.08.013

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

We report the effects of resveratrol, a polyphenol found in the skins of red grapes, on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. Resveratrol suppressed catecholamine secretion and Na-12(+) and Ca-45(2+) influx induced by acetylcholine, an agonist of nicotinic acetylcholine receptors, in a concentration-dependent manner (IC50 = 20.4, 11.0, and 62.8 mu M, respectively). Resveratrol also inhibited catecholamine secretion induced by veratridine, an activator of voltage-dependent Na+ channels, and 56 mM K+, an activator of voltage-dependent Ca2+ channels, at concentrations similar to those for Ca-45(2+) influx. Resveratrol directly inhibited the current evoked by acetylcholine in Xenopus oocytes expressing alpha 3 beta 4 neuronal nicotinic acetylcholine receptors (IC50 = 25.9 mu M). Furthermore, resveratrol (IC50 = 5.32 mu M) attenuated C-14-catecholamine synthesis induced by acetylcholine. The present findings suggest that resveratrol inhibits acetylcholine-induced catecholamine secretion and synthesis through suppressing ion influx in cultured bovine adrenal medullary cells. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：1608 / 1618

页数：11

共 40 条

[1]

Nitric oxide production and endothelium-dependent vasorelaxation induced by wine polyphenols in rat aorta [J].