Specific and rapid quantification of 8-iso-prostaglandin F2α in urine of healthy humans and patients with Zellweger syndrome by gas chromatography tandem mass spectrometry

8-iso-Prostaglandin F-2 alpha (8-iso-PGF(2 alpha)) is currently discussed as a potential index parameter of oxidative stress in vivo. We describe in this article a fully validated gas chromatographic-tandem mass spectrometric method for the quantitative determination of 8-iso-PGF(2 alpha) in human urine. The method is highly specific and requires a single thin-layer chromatographic step for sample purification. Inter- and intraday imprecision were below 8%. Mean inaccuracy was 5.3% for added levels of 8-iso-PGF(2 alpha) up to 2000 pg/ml of urine. We measured highly elevated excretion of 8-iso-PGF(2 alpha) in the urine of children with peroxisomal beta-oxidation deficiency, i.e. Zellweger syndrome, (63.3+/-16.6 ng/mg creatinine) compared to that of healthy children (0.51+/-0.16 ng/mg creatinine) (mean+/-S.D., both n=5). The method could be useful for diagnosing Zellweger syndrome and for investigating the utility of 8-iso-PGF(2 alpha) as a novel marker for oxidative stress in vivo in man. (C) 1998 Elsevier Science B.V. All rights reserved.