Efficient plant regeneration and Agrobacterium-mediated transformation in Medicago and Trifolium species

被引：39

作者：

Ding, YL

Aldao-Humble, G

Ludlow, E

Drayton, M

Lin, YH

Nagel, J

Dupal, M

Zhao, GQ

Pallaghy, C

Kalla, R

Emmerling, M

Spangenberg, G ^{[1
]}

机构：

[1] La Trobe Univ, Ctr Plant Biotechnol, Dept Primary Ind, Bundoora, Vic 3086, Australia

[2] La Trobe Univ, Dept Bot, Bundoora, Vic 3086, Australia

来源：

PLANT SCIENCE | 2003年 / 165卷 / 06期

关键词：

Agrobacterium tumefaciens; transgenic plants; trifolitan species (clovers); Medicago species (alfalfa and medics); cotyledonary explant;

D O I：

10.1016/j.plantsci.2003.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly efficient and robust procedure for plant regeneration from cotyledonary explants was developed for a range of clovers (Trifolium species) including T repens, T pratense, T subterraneum, T michelianum and T isthmocarpum and medics (Medicago species) including alfalfa (M. sativa), M. polymorpha, M. truncatula, M. litoralis and M. tonata. Efficient and reproducible protocols for Agrobacterium-mediated transformation of T repens, T pratense and T subterraneum, using different selectable marker genes, were developed based on the established genotype-independent regeneration procedure. Detailed molecular analysis confirmed the transgenic nature of plants recovered for three clover species tested. Stable meiotic transmission of transgenes was demonstrated for selected transgenic plants carrying single T-DNA inserts. The optimised protocol developed for regeneration and genetic transformation of pasture legumes was refined to demonstrate its application to the production of transgenic and untransformed control plants in otherwise isogenic genetic background from individual pairs of cotyledons. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1419 / 1427

页数：9

共 26 条

[1] CONSTITUTIVE EXPRESSION OF THE BETA-PHASEOLIN GENE IN DIFFERENT TISSUES OF TRANSGENIC ALFALFA DOES NOT ENSURE PHASEOLIN ACCUMULATION IN NONSEED TISSUE [J].