Growing vascular endothelial cells express somatostatin subtype 2 receptors

被引:81
作者
Watson, JC
Balster, DA
Gebhardt, BM
O'Dorisio, TM
O'Dorisio, MS
Espenan, GD
Drouant, GJ
Woltering, EA [1 ]
机构
[1] Louisiana State Univ, Hlth Sci Ctr, Dept Surg, New Orleans, LA 70112 USA
[2] Louisiana State Univ, Hlth Sci Ctr, Dept Ophthalmol, New Orleans, LA 70112 USA
[3] Louisiana State Univ, Hlth Sci Ctr, Dept Radiol, New Orleans, LA 70112 USA
[4] Louisiana State Univ, Hlth Sci Ctr, Stanley S Scott Canc Ctr, New Orleans, LA 70112 USA
[5] Louisiana State Univ, Hlth Sci Ctr, Neurosci Ctr Excellence, New Orleans, LA 70112 USA
[6] Vet Adm Med Ctr, New Orleans, LA 70112 USA
[7] Ohio State Univ, Dept Med, Columbus, OH 43210 USA
[8] Ohio State Univ, Dept Pediat, Columbus, OH 43210 USA
关键词
somatostatin; somatostatin receptors; receptor subtypes; angiogenesis; vascular endothelium; gene expression;
D O I
10.1054/bjoc.2001.1881
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We hypothesized that non-proliferating (quiescent) human vascular endothelial cells would not express somatostatin receptor subtype 2 (sst 2) and that this receptor would be expressed when the endothelial cells begin to grow. To test this hypothesis, placental veins were harvested from 6 human placentas and 2 mm vein disks were cultured in 0.3% fibrin gels. Morphometric analysis confirmed that 50-75% of cultured vein disks developed radial capillary growth within 15 days. Sst 2 gene expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) analysis of the RNA from veins before culture and from tissue-matched vein disks that exhibited an angiogenic response. The sst 2 gene was expressed in the proliferating angiogenic sprouts of human vascular endothelium. The presence of sst 2 receptors on proliferating angiogenic vessels was confirmed by immunohistochemical staining and in vivo scintigraphy. These results suggest that sst 2 may be a unique target for antiangiogenic therapy with sst 2 preferring somatostatin analogues conjugated to radioisotopes or cytotoxic agents. (C) 2001 Cancer Research Campaign.
引用
收藏
页码:266 / 272
页数:7
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