Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE)

被引:85
作者
Deutsch, Eric W. [1 ]
Ball, Catherine A. [2 ]
Berman, Jules J. [3 ]
Bova, G. Steven [4 ,5 ,6 ,7 ,8 ]
Brazma, Alvis [9 ]
Bumgarner, Roger E. [10 ]
Campbell, David [1 ]
Causton, Helen C. [11 ]
Christiansen, Jeffrey H. [12 ]
Daian, Fabrice [13 ]
Dauga, Delphine [13 ]
Davidson, Duncan R. [12 ]
Gimenez, Gregory [13 ]
Goo, Young Ah [1 ,14 ]
Grimmond, Sean [15 ]
Henrich, Thorsten [16 ]
Herrmann, Bernhard G. [17 ,18 ]
Johnson, Michael H. [1 ]
Korb, Martin [1 ]
Mills, Jason C. [19 ]
Oudes, Asa J. [1 ,14 ]
Parkinson, Helen E.
Pascal, Laura E. [1 ,14 ]
Pollet, N. Icolas [20 ]
Quackenbush, John [21 ]
Ramialison, Mirana [16 ]
Ringwald, Martin [22 ]
Salgado, David [13 ]
Sansone, Susanna-Assunta [9 ]
Sherlock, Gavin [23 ]
Stoeckert, Christian J., Jr. [24 ,25 ]
Swedlow, Jason [26 ]
Taylor, Ronald C. [27 ]
Walashek, Laura [1 ,14 ]
Warford, Anthony [28 ]
Wilkinson, David G. [29 ]
Zhou, Yi [30 ,31 ]
Zon, Leonard I. [30 ,31 ]
Liu, Alvin Y. [1 ,14 ]
True, Lawrence D. [32 ]
机构
[1] Inst Syst Biol, 1441 N 34th St, Seattle, WA 98103 USA
[2] Stanford Univ, Sch Med, Dept Biochem, Stanford, CA 94305 USA
[3] Assoc Pathol Informat, Bethesda, MD 20814 USA
[4] Johns Hopkins Univ, Sch Med, PELICAN Lab, Dept Pathol, Baltimore, MD 21287 USA
[5] Johns Hopkins Univ, Sch Med, PELICAN Lab, Dept Hlth Informat Sci, Baltimore, MD 21287 USA
[6] Johns Hopkins Univ, Sch Med, PELICAN Lab, Dept Med Genet, Baltimore, MD 21287 USA
[7] Johns Hopkins Univ, Sch Med, PELICAN Lab, Dept Oncol, Baltimore, MD 21287 USA
[8] Johns Hopkins Univ, Sch Med, PELICAN Lab, Dept Urol, Baltimore, MD 21287 USA
[9] European Bioinformat Inst, European Mol Biol Lab, Cambridge CB10 1SD, England
[10] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA
[11] Imperial Coll Sch Med, MRC, Clin Sci Ctr Microarray Ctr, London W12 0NN, England
[12] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
[13] Univ Mediterranee, CNRS, Inst Biol Dev Marseille, UMR 6216, F-13288 Marseille 9, France
[14] Univ Washington, Dept Urol, Seattle, WA 98195 USA
[15] Univ Queensland, Inst Mol Biosci, St Lucia, Qld 4027, Australia
[16] EMBL Heidelberg, D-69117 Heidelberg, Germany
[17] Max Planck Inst Mol Genet, Inst Med Genet, D-14195 Berlin, Germany
[18] Max Planck Inst Mol Genet, Dept Dev Genet, D-14195 Berlin, Germany
[19] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA
[20] Univ Paris 11, CNRS, UMR 8080, F-91405 Orsay, France
[21] Dana Farber Canc Inst, Boston, MA 02115 USA
[22] Jackson Lab, Bar Harbor, ME 04609 USA
[23] Stanford Univ, Dept Genet, Sch Med, Stanford, CA 94305 USA
[24] Univ Penn, Ctr Bioinformat, Philadelphia, PA 19104 USA
[25] Univ Penn, Dept Genet, Philadelphia, PA 19104 USA
[26] Univ Dundee, Div Gene Regulat & Express, Wellcome Trust Bioctr, Dundee DD1 5EH, Scotland
[27] Pacific NW Natl Lab, Computat Biol & Bioinformat Grp, Richland, WA 99352 USA
[28] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England
[29] Natl Inst Med Res, Div Dev Neurobiol, London NW7 1AA, England
[30] Childrens Hosp, Div Hematol Oncol, Stem Cell Program, Boston, MA 02115 USA
[31] Harvard Univ, Sch Med, Boston, MA 02115 USA
[32] Univ Washington, Dept Pathol, Seattle, WA 98195 USA
基金
英国医学研究理事会; 英国生物技术与生命科学研究理事会;
关键词
D O I
10.1038/nbt1391
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.
引用
收藏
页码:305 / 312
页数:8
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