Molecular diversity and evolution of cystine knot toxins of the tarantula Chilobrachys jingzhao

被引：61

作者：

Chen, J. ^{[1
]}

Deng, M. ^{[1
]}

He, Q. ^{[1
]}

Meng, E. ^{[1
]}

Jiang, L. ^{[1
]}

Liao, Z. ^{[1
]}

Rong, M. ^{[1
]}

Liang, S. ^{[1
]}

机构：

[1] Hunan Normal Univ, Key Lab Prot Chem & Dev Biol, Minist Educ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2008年 / 65卷 / 15期

关键词：

toxin; molecular diversity; classification; pore-blocking; gating modifier; evolution;

D O I：

10.1007/s00018-008-8135-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cystine knot toxins (CKTs) in spider venoms represent a rich source of novel ligands for varied ion channels. Here, we identified 95 novel putative CKT precursors by analyzing expressed sequence tags of the tarantula Chilobrachys jingzhao venom gland. Phylogenetics analyses revealed one orphan family and six families with sequence similarity to known toxins. To further investigate the relationships of their structures, functions and evolution, we assayed 10 representative toxins for their effect on ion channels, and performed structure model comparisons, evolution analysis and toxin distribution analysis. This study revealed two major types of CKTs: pore-blocking toxins and gating modifier toxins. A few blockers were observed with relatively high abundance and wide distribution, which may be a category of original toxins that block channels conserved in various preys with relatively high specificity. The gating modifier families contain advanced toxins, usually have many members and interact with diverse regulatory components of channels.

引用

页码：2431 / 2444

页数：14

共 58 条

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