Barcode lateral flow immunochromatographic strip for prostate acid phosphatase determination

被引：44

作者：

Fang, Cheng ^{[1
,2
]}

Chen, Zhencheng ^{[2
]}

Li, Lin ^{[2
]}

Xia, Jinhong ^{[2
]}

机构：

[1] Cent S Univ, Dept Biomed Engn, Sch Geosci & Infophys, Changsha 410083, Hunan, Peoples R China

[2] Guilin Univ Elect Technol, Sch Life & Environm Sci, Guilin 541004, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2011年 / 56卷 / 05期

基金：

国家高技术研究发展计划(863计划);

关键词：

Barcode; Immunochromatographic strip; Prostate acid phosphatase; Enzyme linked immunosorbent assay; SEMIQUANTITATIVE DETECTION; ASSAY; IMMUNOASSAY; CANCER; AGREEMENT; PROTEIN;

D O I：

10.1016/j.jpba.2011.08.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A barcode semiquantitative lateral flow immunochromatographic strip for prostate acid phosphatase (PAP) was developed, in which the monoclonal antibody specific for PAP was labeled to gold nanoparticle and another monoclonal antibody was immobilized on nitrocellulose membrane in the barcode fashion respectively. Based on the stepwise capture of analyte, the system expresses the concentration of PAP in nanogram range as four distinct ladder bars in 30 min, therefore, which could be detected directly by naked eye or image analyzer. Serum PAP from 65 patients was detected with this method and compared with enzyme linked immunosorbent assay (ELISA). There is a good agreement between the methods. Its easily readable result, and also its simplicity and low cost offers an alternative for testing PAP. By incorporating with different specific antibody, the assay can be further extended to detect a variety of analytes with clinical importance. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：1035 / 1040

页数：6

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