Embryonic stem cells are capable of generating a neuronal network in the adult mouse retina

被引：22

作者：

Hara, A

Niwa, M

Kunisada, T

Yoshimura, N

Katayama, M

Kozawa, O

Mori, H

机构：

[1] Gifu Univ, Sch Med, Dept Tumor Pathol, Gifu 5008705, Japan

[2] Gifu Univ, Sch Med, Dept Pharmacol, Gifu 5008705, Japan

[3] Gifu Univ, Sch Med, Med Educ Dev Ctr, Gifu 5008705, Japan

[4] Gifu Univ, Grad Sch Med, Dept Tissue & Organ Dev, Gifu 5008705, Japan

来源：

BRAIN RESEARCH | 2004年 / 999卷 / 02期

关键词：

embryonic stem cell; neuronal network; adult mouse retina;

D O I：

10.1016/j.brainres.2003.11.055

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The integration of embryonic stem (ES) cells as well-differentiated neuronal cells into the retinas of adult mice was investigated. ES cells were transplanted in adult mouse retinas by intravitreal injections. Neuronal differentiation of the ES cells was investigated by morphological and immunohistochemical examinations on post-operative days 5 and 30. ES cell apoptosis was examined by in situ terminal dUTP-biotin nick end labeling of DNA fragments. Differentiated ES cells growing along the retinal surface developed fine neuronal cell processes around cell nuclei and generated neuronal networks into the retinal inner plexiform layer (IPL) 30 days after transplantation. The differentiated ES cells expressed retinal and neuronal markers. Many apoptotic cells were recognized in transplanted ES cells at day 5 but not at day 30 after transplantation. ES cells may be useful for neural tissue regeneration in the adult mammalian retina. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：216 / 221

页数：6

共 22 条

[1] PATTERNS OF CELL-PROLIFERATION IN THE DEVELOPING RETINA OF THE CLAWED FROG IN RELATION TO BLOOD-SUPPLY AND POSITION OF THE CHOROIDAL FISSURE [J].