Commercial riboflavin production by recombinant Bacillus subtilis:: down-stream processing and comparison of the composition of riboflavin produced by fermentation or chemical synthesis

被引：26

作者：

Bretzel, W ^{[1
]}

Schurter, W ^{[1
]}

Ludwig, B ^{[1
]}

Kupfer, E ^{[1
]}

Doswald, S ^{[1
]}

Pfister, M ^{[1
]}

van Loon, APGM ^{[1
]}

机构：

[1] F Hoffmann La Roche Ltd, Biotechnol Vitamins & Fine Chem Div, CH-4070 Basel, Switzerland

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1999年 / 22卷 / 01期

关键词：

recombinant Bacillus subtilis; riboflavin produced by fermentation; down-stream processing; analytics; registration;

D O I：

10.1038/sj.jim.2900604

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel process for riboflavin production using a recombinant Bacillus subtilis strain has been developed. Here we describe a down-stream processing procedure to obtain riboflavin qualities having a minimal content of 96% ('feed-grade') and 98% ('food/pharma-grade') riboflavin, respectively, Compared to riboflavin produced by chemical synthesis, products with improved chemical purity were obtained, All compounds representing more than 0.1% of the final products were identified. Feed-grade riboflavin material ex fermentation contained small amounts of amino acids and amino sugars and the biosynthetic riboflavin precursor dimethyl-ribityl-lumazine, All other side products found were derived from riboflavin, resulted from the purification procedure and were also found in riboflavin obtained by chemical synthesis, The Bacillus-produced riboflavin does not contain DNA. The data presented here were used to obtain product approval for the commercial application in the USA, Japan and the UK.

引用

页码：19 / 26

页数：8

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