Use of antiserum-coated latex particles for serotyping Streptococcus pneumoniae

被引:29
作者
Arai, S
Konda, T
Wada, A
Matsunaga, Y
Okabe, N
Watanabe, H
Inouye, S
机构
[1] Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Tokyo 1628640, Japan
[2] Natl Inst Infect Dis, Infect Dis Surveillance Ctr, Shinjuku Ku, Tokyo 1628640, Japan
[3] Natl Inst Infect Dis, Dept Bacterial & Blood Prod, Tokyo 2080011, Japan
关键词
anti-rabbit IgG-coated latex particles; protein A-coated latex particles; latex agglutination; Streptococcus pneumoniae serotyping/serogrouping;
D O I
10.1111/j.1348-0421.2001.tb01284.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The Quellung reaction provides a standard means for serotyping Streptococcus pneumoniae, but it requires microscopic examination with skillful technique, We have developed an improved agglutination method with anti-rabbit IgG-coated latex particles, which are sensitized with pooled antisera for serotyping/serogrouping S. pneumoniae. Our method is as specific and sensitive as the Quellung test, and much easier to perform.
引用
收藏
页码:159 / 162
页数:4
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