Influence of Agrobacterium tumefaciens strain on the production of transgenic peas (Pisum sativum L.)

被引：18

作者：

Grant, JE ^{[1
]}

Thomson, LMJ ^{[1
]}

Pither-Joyce, MD ^{[1
]}

Dale, TM ^{[1
]}

Cooper, PA ^{[1
]}

机构：

[1] New Zealand Inst Crop & Food Res, Christchurch, New Zealand

来源：

PLANT CELL REPORTS | 2003年 / 21卷 / 12期

关键词：

pea transformation efficiency; Agrobacterium strain; Pisum sativum; transgenic peas;

D O I：

10.1007/s00299-003-0640-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We compared the efficiency of two Agrobacterium tumefaciens strains, AGL 1 and KYRT1, for producing transgenic pea plants. KYRT1 is a disarmed strain of Chry5 that has been shown to be highly tumourigenic on soybean. The efficacies of the strains were compared using cotyledon explants from three pea genotypes and two plasmids. The peas were sourced from field-grown plants over three Southern Hemisphere summer seasons. Overall, KYRT1 was found to be on average threefold more efficient than AGL 1 for producing transgenic plants. We suggest that KYRT1 is sensitive to cocultivation temperature as the expected increase in efficiency was not achieved at high laboratory temperatures.

引用

页码：1207 / 1210

页数：4

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