Clearance of protoporphyrin IX from mouse skin after topical application of 5-aminolevulinic acid and its methyl ester

被引：6

作者：

Juzenas, P ^{[1
]}

Sorensen, R ^{[1
]}

Iani, V ^{[1
]}

Moan, J ^{[1
]}

机构：

[1] Norwegian Radium Hosp, Inst Canc Res, Dept Biophys, N-0310 Oslo, Norway

来源：

PHOTOHEMOTHERAPY OF CANCER AND OTHER DISEASES, PROCEEDINGS | 1999年 / 3563卷

关键词：

5-aminolevulinic acid (ALA); 5-aminolevulinic acid methyl ester (ALA-Me); protoporphyrin IX (PpIX); topical application;

D O I：

10.1117/12.339134

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The clearance of protoporphyrin IX (PpIX) from the skin of hairless BALB/c mice after topical application of 5-aminolevulinic acid (ALA) and its methyl ester (ALA-Me) was investigated. Creams containing 2 or 20% of ALA or ALA-Me were topically applied on spots of approximately 1 cm(2) for 12 hours. The PpIX fluorescence was detected by the means of a Perkin Elmer LS50B luminescence spectrometer equipped with a fibre-optic probe. The emission spectrum was identical with that of cell-bound PpIX. After 12 hours application of ALA and ALA-Me similar amounts of PpIX were found. After cream removal the ALA-induced PpIX fluorescence decayed with a half-life of about 20 hours (20% ALA cream). The ALA-Me-induced PpIX was faster cleared from the skin than ALA-induced PpIX, and had a half-life of about 7 hours (20% ALA-Me cream).

引用

页码：161 / 166

页数：6

共 17 条

[1] SUPERFICIAL PHOTODYNAMIC THERAPY WITH TOPICAL 5-AMINOLEVULINIC ACID FOR SUPERFICIAL PRIMARY AND SECONDARY SKIN-CANCER [J].

CAIRNDUFF, F ;

STRINGER, MR ;

HUDSON, EJ ;

ASH, DV ;

BROWN, SB .

BRITISH JOURNAL OF CANCER, 1994, 69 (03) :605-608

[2] PORPHYRIN METABOLISM IN SOME MALIGNANT DISEASES [J].

ELSHARABASY, MMH ;

ELWASEEF, AM ;

HAFEZ, MM ;

SALIM, SA .

BRITISH JOURNAL OF CANCER, 1992, 65 (03) :409-412

[3] Primary clinical response and long-term follow-up of solar keratoses treated with topically applied 5-aminolevulinic acid and irradiation by different wave bands of light [J].

Fink-Puches, R ;

Hofer, A ;

Smolle, J ;

Kerl, H ;

Wolf, P .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 41 (1-2) :145-151

[4]

Gaullier JM, 1997, CANCER RES, V57, P1481

[5] PHOTOSENSITIZATION OF MURINE TUMOR, VASCULATURE AND SKIN BY 5-AMINOLEVULINIC ACID-INDUCED PORPHYRIN [J].

HENDERSON, BW ;

VAUGHAN, L ;

BELLNIER, DA ;

VANLEENGOED, H ;

JOHNSON, PG ;

OSEROFF, AR .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1995, 62 (04) :780-789

[6] ENDOGENOUS PROTOPORPHYRIN-IX, A CLINICALLY USEFUL PHOTOSENSITIZER FOR PHOTODYNAMIC THERAPY [J].

KENNEDY, JC ;

POTTIER, RH .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1992, 14 (04) :275-292

[7] PHOTODYNAMIC THERAPY WITH ENDOGENOUS PROTOPORPHYRIN .9. BASIC PRINCIPLES AND PRESENT CLINICAL-EXPERIENCE [J].

KENNEDY, JC ;

POTTIER, RH ;

PROSS, DC .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1990, 6 (1-2) :143-148

[8] TOPICAL APPLICATION OF 5-AMINOLEVULINIC ACID, DMSO AND EDTA - PROTOPORPHYRIN-IX ACCUMULATION IN SKIN AND TUMORS OF MICE [J].

MALIK, Z ;

KOSTENICH, G ;

ROITMAN, L ;

EHRENBERG, B ;

ORENSTEIN, A .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1995, 28 (03) :213-218

[9] Clinical results of photodynamic therapy for superficial skin malignancies or actinic keratosis using topical 5-aminolaevulinic acid [J].

Meijnders, PJN ;

Star, WM ;

DeBruijn, RS ;

TreurnietDonker, AD ;

VanMierlo, MJM ;

Wijthoff, SJM ;

Naafs, B ;

Beerman, H ;

Levendag, PC .

LASERS IN MEDICAL SCIENCE, 1996, 11 (02) :123-131

[10] TEMPERATURE MONITORING DURING PHOTODYNAMIC THERAPY OF SKIN TUMORS WITH TOPICAL 5-AMINOLEVULINIC ACID APPLICATION [J].

ORENSTEIN, A ;

KOSTENICH, G ;

TSUR, H ;

KOGAN, L ;

MALIK, Z .

CANCER LETTERS, 1995, 93 (02) :227-232

← 1 2 →