Expression profiling in cancer using cDNA microarrays

被引：5

作者：

Khan, J ^{[1
]}

Saal, LH ^{[1
]}

Bittner, ML ^{[1
]}

Chen, YD ^{[1
]}

Trent, JM ^{[1
]}

Meltzer, PS ^{[1
]}

机构：

[1] Natl Human Genome Res Inst, Canc Genet Branch, NIH, Bethesda, MD 20892 USA

来源：

ELECTROPHORESIS | 1999年 / 20卷 / 02期

关键词：

cDNA microarray; gene expression; cancer research; review;

D O I：

10.1002/(SICI)1522-2683(19990201)20:2<223::AID-ELPS223>3.0.CO;2-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently there are over 1 000 000 human expressed sequence tag (EST) sequences available on the public database, representing perhaps 50-90% of all human genes. The cDNA microarray technique is a recently developed tool that exploits this wealth of information for the analysis of gene expression. In this method, DNA probes representing cDNA clones are arrayed onto a glass slide and interrogated with fluorescently labeled cDNA targets. The power: of the technology is the ability to perform a genome-wide expression profile of thousands of genes in one experiment. In our review we describe the principles of the microarray technology as applied to cancer research, summarize the literature on its use so far, and speculate on the future application of this powerful technique.

引用

页码：223 / 229

页数：7

共 19 条

[1] ESTABLISHING A HUMAN TRANSCRIPT MAP [J].

BOGUSKI, MS ;

SCHULER, GD .

NATURE GENETICS, 1995, 10 (04) :369-371

[2]

CHEN Y, 1997, BIOMED OPTICS, V2, P364

[3]

DeRisi J, 1996, NAT GENET, V14, P457

[4] Exploring the metabolic and genetic control of gene expression on a genomic scale [J].

DeRisi, JL ;

Iyer, VR ;

Brown, PO .

SCIENCE, 1997, 278 (5338) :680-686

[5] Gene-representing cDNA clusters defined by hybridization of 57,419 clones from infant brain libraries with short oligonucleotide probes [J].

Drmanac, S ;

Stavropoulos, NA ;

Labat, I ;

Vonau, J ;

Hauser, B ;

Soares, MB ;

Drmanac, R .

GENOMICS, 1996, 37 (01) :29-40

[6]

DRMANAC S, 1974, BIOTECHNIQUES, V328, P332

[7] Data management and analysis for gene expression arrays [J].

Ermolaeva, O ;

Rastogi, M ;

Pruitt, KD ;

Schuler, GD ;

Bittner, ML ;

Chen, YD ;

Simon, R ;

Meltzer, P ;

Trent, JM ;

Boguski, MS .

NATURE GENETICS, 1998, 20 (01) :19-23

[8] HOW IS THE HUMAN GENOME PROJECT DOING, AND WHAT HAVE WE LEARNED SO FAR [J].

GUYER, MS ;

COLLINS, FS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :10841-10848

[9] Discovery and analysis of inflammatory disease-related genes using cDNA microarrays [J].

Heller, RA ;

Schena, M ;

Chai, A ;

Shalon, D ;

Bedilion, T ;

Gilmore, J ;

Woolley, DE ;

Davis, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2150-2155

[10]

Khan J, 1998, CANCER RES, V58, P5009

← 1 2 →