Cloning and sequencing of a new 15-hydroxyprostaglandin dehydrogenase related mRNA

被引：11

作者：

DelageMourroux, R ^{[1
]}

Pichaud, F ^{[1
]}

Frendo, JL ^{[1
]}

Pidoux, E ^{[1
]}

Guliana, JM ^{[1
]}

Moukhtar, MS ^{[1
]}

Jullienne, A ^{[1
]}

机构：

[1] HOP LARIBOISIERE,INSERM,U349,CTR VIGGO PETERSEN,F-75475 PARIS 10,FRANCE

来源：

GENE | 1997年 / 188卷 / 01期

关键词：

TT cell line; medullary thyroid carcinoma; RT-PCR; nucleic sequence; ribonuclease protection;

D O I：

10.1016/S0378-1119(96)00800-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (type-I 15-PGDH) inactivates prostaglandins. We recently reported an mRNA sequence coding for a predicted isomer (PGDH(rI)) of this enzyme. The TT cell line, derived from medullary thyroid carcinoma (MTC), expresses mRNAs for both isomers. We report here the expression by TT cells and MTC of a third 15-PGDH related mRNA (PGDH(rII)), 241 nt shorter than type-I 15-PGDH. RNase protection assays confirmed that TT cells expressed this mRNA (PGDH(rII)). Thus different splicing patterns could be involved in the post-transcriptional regulation of type-I 15-PGDH gene in MTC.

引用

页码：143 / 148

页数：6

共 11 条

[1] BIOLOGICAL ACTIVITIES OF 3 METABOLITES OF PROSTAGLANDIN E1
ANGGARD, E
[J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1966, 66 (04): : 509 - &
[2] ANGGARD E, 1964, J BIOL CHEM, V239, P4097
[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION
CHOMCZYNSKI, P
SACCHI, N
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) : 156 - 159
[4] DESSEN P, 1990, COMPUT APPL BIOSCI, V6, P355
[5] BACTERIAL EXPRESSION AND SITE-DIRECTED MUTAGENESIS OF 2 CRITICAL RESIDUES (TYROSINE-151 AND LYSINE-155) OF HUMAN PLACENTAL NAD(+)-DEPENDENT 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE
ENSOR, CM
TAI, HH
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1994, 1208 (01): : 151 - 156
[6] ENSOR CM, 1990, J BIOL CHEM, V265, P14888
[7] PURIFICATION AND STRUCTURAL CHARACTERIZATION OF PLACENTAL NAD+-LINKED 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE - THE PRIMARY STRUCTURE REVEALS THE ENZYME TO BELONG TO THE SHORT-CHAIN ALCOHOL-DEHYDROGENASE FAMILY
KROOK, M
MAREKOV, L
JORNVALL, H
[J]. BIOCHEMISTRY, 1990, 29 (03) : 738 - 743
[8] LEONG SS, 1981, FIELD ED ITALIA, P95
[9] CHARACTERISTICS OF SHORT-CHAIN ALCOHOL DEHYDROGENASES AND RELATED ENZYMES
PERSSON, B
KROOK, M
JORNVALL, H
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 200 (02): : 537 - 543
[10] SEQUENCE OF A NOVEL MESSENGER-RNA CODING FOR A C-TERMINAL-TRUNCATED FORM OF HUMAN NAD(+)-DEPENDENT 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE
PICHAUD, F
FRENDO, JL
DELAGEMOURROUX, R
DEVERNEJOUL, MC
MOUKHTAR, MS
JULLIENNE, A
[J]. GENE, 1995, 162 (02) : 319 - 322

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