A new intracellular serine protease inhibitor expressed in the rat pituitary gland complexes with granzyme B

被引：6

作者：

Hill, RM

Morresey, KS

Coates, LC

Mezey, E

Fell, B

Bratt, T

Trapani, JA

Birch, NP

机构：

[1] Univ Auckland, Sch Biol Sci, Mol Neuroendocrinol Grp, Auckland 1, New Zealand

[2] NINDS, Basic Neurosci Program, NIH, Bethesda, MD 20892 USA

[3] Austin Res Inst, Cellular Cytotox Lab, Heidelberg, Vic, Australia

来源：

FEBS LETTERS | 1998年 / 440卷 / 03期

关键词：

inhibitor; pituitary; serpin; serine protease; granzyme B;

D O I：

10.1016/S0014-5793(98)01475-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned a novel serpin (raPIT5a) from a rat pituitary cDNA library which is structurally related to members of the ovalbumin subfamily of serine protease inhibitors. This new cDNA encodes a 374-amino acid protein, designated raPIT5a. raPIT5a was expressed in specific cells in the intermediate and anterior lobes of the pituitary. Recombinant raPIT5a was not secreted suggesting raPIT5a functions to inhibit intracellular proteases, Recombinant raPIT5a formed an SDS-stable complex with human granzyme B, a serine protease which induces apoptosis by activating members of the caspase enzyme family. These data suggest raPIT5a may have a role in regulating granzyme B or related enzymes and apoptosis in the pituitary gland. (C) 1998 Federation of European Biochemical Societies.

引用

页码：361 / 364

页数：4

共 25 条

[1] NATURAL PROTEIN PROTEINASE-INHIBITORS AND THEIR INTERACTION WITH PROTEINASES [J].