Expression of lymphotoxin gene inserted into Theiler's murine encephalomyelitis virus

被引：3

作者：

Obuchi, M

Odagiri, T

Iizuka, H

Ohara, Y ^{[1
]}

机构：

[1] Kanazawa Med Univ, Dept Microbiol, Uchinada, Ishikawa 9200293, Japan

[2] Kanazawa Med Univ, Dept Neurosurg, Uchinada, Ishikawa 9200293, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1999年 / 43卷 / 01期

关键词：

TMEV; viral vector; lymphotoxin;

D O I：

10.1111/j.1348-0421.1999.tb02377.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non-fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as a vector for the introduction of a foreign gene into the central nervous system, Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme-linked immunosorbent assay on BHK-21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells.

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页码：83 / 86

页数：4

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