Functional regulation of Gα16 by protein kinase C

被引:22
作者
Aragay, AM
Quick, MW
机构
[1] Univ Alabama, Dept Neurobiol, Birmingham, AL 35294 USA
[2] Univ Autonoma Madrid, Fac Ciencias, Ctr Biol Mol, E-28049 Madrid, Spain
关键词
D O I
10.1074/jbc.274.8.4807
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent evidence demonstrates that the a subunits of some heterotrimeric GTP-binding proteins (G proteins) are subject to modification by protein kinase C (PKC). For the family of G proteins that activate the phospholipase C/inositol trisphosphate/calcium/PKC pathway, such modification could result in G protein autoregulation. To examine the potential regulation of members of the G alpha(q) family by PKC phosphorylation, we expressed the thyrotropin-releasing hormone (TRH) receptor in combination with G alpha(q), G alpha(11), G alpha(14), G alpha(15), or G alpha(16) in Xenopus oocytes and examined the regulation of signaling by PKC activators and inhibitors, For G alpha(16) and G alpha(15), the two family members of hematopoietic lineage, PKC activators reduce both the magnitude and the time course of TRH-mediated responses; PKC inhibitors have the opposite effect. The PKC-mediated effects are evident in measurements of GTPase activity, suggesting that the regulation is occurring early in the signaling pathway. In vivo phosphorylation experiments demonstrate that G alpha(16) is a substrate for PHC modification. By comparison, G alpha(q) is not phosphorylated by PHC in vivo and oocytes expressing G alpha(q) are not functionally modulated by PKC. Repeated TRH stimulation of oocytes expressing G alpha(16) mimics the effects of PKC activators, and this functional regulation is correlated with an increase in G alpha(16) phosphorylation. A mutant G alpha(16) with four consensus PKC phosphorylation sites removed is not phosphorylated in vivo, and TRH responses mediated through the mutant are not regulated by PKC. These results demonstrate that signaling involving hematopoietic G proteins is subject to PKC-mediated autoregulation, at least in part, by phosphorylation of the G protein a subunit.
引用
收藏
页码:4807 / 4815
页数:9
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