Chemo-enzymatic synthesis of the Galili epitope Galα(1→3)Galβ(1→4)GlcNAc on a homogeneously soluble PEG polymer by a multi-enzyme system

被引：36

作者：

Brinkmann, N

Malissard, M

Ramuz, M

Römer, U

Schumacher, T

Berger, EG

Elling, L

Wandrey, C

Liese, A

机构：

[1] Forschungszentrum Julich, Inst Biotechnol, D-52425 Julich, Germany

[2] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland

[3] Univ Duesseldorf, Inst Enzyme Technol, D-52426 Julich, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2001年 / 11卷 / 18期

关键词：

D O I：

10.1016/S0960-894X(01)00474-7

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The alpha -Gal trisaccharide Gal alpha (1 -->3)Gal beta (1 -->4)GlcNAc 11 was synthesized on a homogeneously soluble polymeric support (polyethylene glycol, PEG) by use of a multi-enzyme system consisting of beta -1,4-galactosyltransferase (EC 2.4.1.38), alpha -1,3-galactosyltransferase (EC 2.4.1.151), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2). In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free alpha -Gal trisaccharide. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2503 / 2506

页数：4

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[1] Searching for medicine's sweet spot [J].