Involvement of cathepsin B in the plant disease resistance hypersensitive response

被引:131
作者
Gilroy, Eleanor M.
Hein, Ingo
van der Hoorn, Renier
Boevink, Petra C.
Venter, Eduard
McLellan, Hazel
Kaffarnik, Florian
Hrubikova, Katarina
Shaw, Jane
Holeva, Maria
Lopez, Eduardo C.
Borras-Hidalgo, Orlando
Pritchard, Leighton
Loake, Gary J.
Lacomme, Christophe
Birch, Paul R. J. [1 ]
机构
[1] Scottish Crop Res Inst, Plant Pathol Programme, Dundee DD2 5DA, Scotland
[2] Max Planck Inst Plant Breeding Res, D-50829 Cologne, Germany
[3] Univ Edinburgh, Sch Biol Sci, Inst Mol Plant Sci, Edinburgh EH9 3JR, Midlothian, Scotland
[4] John Innes Ctr, Sainsbury Lab, Norwich NR5 7UH, Norfolk, England
[5] Ctr Genet Engn & Biotechnol, Havana 10600, Cuba
关键词
papain; apoptosis; protease; non-host; gene-for-gene; PROGRAMMED CELL-DEATH; FUNCTIONAL-ANALYSIS; CYSTEINE PROTEASES; PROTEIN; ARABIDOPSIS; APOPTOSIS; TOBACCO; GENES; SGT1; IDENTIFICATION;
D O I
10.1111/j.1365-313X.2007.03226.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A diverse range of plant proteases are implicated in pathogen perception and in subsequent signalling and execution of disease resistance. We demonstrate, using protease inhibitors and virus-induced gene silencing (VIGS), that the plant papain cysteine protease cathepsin B is required for the disease resistance hypersensitive response (HR). VIGS of cathepsin B prevented programmed cell death (PCD) and compromised disease resistance induced by two distinct non-host bacterial pathogens. It also suppressed the HR triggered by transient co-expression of potato R3a and Phytophthora infestans Avr3a genes. However, VIGS of cathepsin B did not compromise HR following recognition of Cladosporium fulvum AVR4 by tomato Cf-4, indicating that plant PCD can be independent of cathepsin B. The non-host HR to Erwinia amylovora was accompanied by a transient increase in cathepsin B transcript level and enzymatic activity and induction of the HR marker gene Hsr203. VIGS of cathepsin B significantly reduced the induction of Hsr203 following E. amylovora challenge, further demonstrating a role for this protease in PCD. Whereas cathepsin B is often relocalized from the lysosome to the cytosol during animal PCD, plant cathepsin B is secreted into the apoplast, and is activated upon secretion in the absence of pathogen challenge.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 49 条
[1]   Type III secretion system effector proteins: Double agents in bacterial disease and plant defense [J].
Alfano, JR ;
Collmer, A .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2004, 42 :385-414
[2]   An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm [J].
Armstrong, MR ;
Whisson, SC ;
Pritchard, L ;
Bos, JIB ;
Venter, E ;
Avrova, AO ;
Rehmany, AP ;
Böhme, U ;
Brooks, K ;
Cherevach, I ;
Hamlin, N ;
White, B ;
Frasers, A ;
Lord, A ;
Quail, MA ;
Churcher, C ;
Hall, N ;
Berriman, M ;
Huang, S ;
Kamoun, S ;
Beynon, JL ;
Birch, PRJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (21) :7766-7771
[3]   Potato oxysterol binding protein and cathepsin B are rapidly up-regulated in independent defence pathways that distinguish R gene-mediated and field resistances to Phytophthora infestans [J].
Avrova, AO ;
Taleb, N ;
Rokka, VM ;
Heilbronn, J ;
Campbell, E ;
Hein, I ;
Gilroy, EM ;
Cardle, L ;
Bradshaw, JE ;
Stewart, HE ;
Fakim, YJ ;
Loake, G ;
Birch, PRJ .
MOLECULAR PLANT PATHOLOGY, 2004, 5 (01) :45-56
[4]   Gene expression in autumn leaves [J].
Bhalerao, R ;
Keskitalo, J ;
Sterky, F ;
Erlandsson, R ;
Björkbacka, H ;
Birve, SJ ;
Karlsson, J ;
Gardeström, P ;
Gustafsson, P ;
Lundeberg, J ;
Jansson, S .
PLANT PHYSIOLOGY, 2003, 131 (02) :430-442
[5]   The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana [J].
Bos, Jorunn I. B. ;
Kanneganti, Thirumala-Devi ;
Young, Carolyn ;
Cakir, Cahid ;
Huitema, Edgar ;
Win, Joe ;
Armstrong, Miles R. ;
Birch, Paul R. J. ;
Kamoun, Sophien .
PLANT JOURNAL, 2006, 48 (02) :165-176
[6]   A monomeric red fluorescent protein [J].
Campbell, RE ;
Tour, O ;
Palmer, AE ;
Steinbach, PA ;
Baird, GS ;
Zacharias, DA ;
Tsien, RY .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882
[7]  
CAO H, 1994, PLANT CELL, V6, P1583, DOI 10.1105/tpc.6.11.1583
[8]   A plant caspase-like protease activated during the hypersensitive response [J].
Chichkova, NV ;
Kim, SH ;
Titova, ES ;
Kalkum, M ;
Morozov, VS ;
Rubtsov, YP ;
Kalinina, NO ;
Taliansky, ME ;
Vartapetian, AB .
PLANT CELL, 2004, 16 (01) :157-171
[9]   Purification and characterization of serine proteases that exhibit caspase-like activity and are associated with programmed cell death in Avena sativa [J].
Coffeen, WC ;
Wolpert, TJ .
PLANT CELL, 2004, 16 (04) :857-873
[10]   A gateway cloning vector set for high-throughput functional analysis of genes in planta [J].
Curtis, MD ;
Grossniklaus, U .
PLANT PHYSIOLOGY, 2003, 133 (02) :462-469