Increased levels of cathepsin B and L, urokinase-type plasminogen activator and its inhibitor type-1 as an early event in gastric carcinogenesis

被引:32
作者
Farinati, F
Herszenyi, L
Plebani, M
Carraro, P
DePaoli, M
Cardin, R
Roveroni, G
Rugge, M
Nitti, D
Grigioni, WF
DErrico, A
Naccarato, R
机构
[1] UNIV PADUA, IST MED INTERNA, CATTEDRA MALATTIE APPARATO DIGERENTE, I-35100 PADUA, ITALY
[2] UNIV PADUA, CATTEDRA ISTOCHIM & IMMUNOISTOCHIM PATOL, IST ANAT PATOL, I-35100 PADUA, ITALY
[3] UNIV PADUA, IST CLIN CHIRURG 2, I-35100 PADUA, ITALY
[4] UNIV BOLOGNA, IST ANAT PATOL, BOLOGNA, ITALY
[5] UNIV PADUA, DIPARTIMENTO MED LAB, CENT LAB, PADUA, ITALY
关键词
D O I
10.1093/carcin/17.12.2581
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Cysteine proteases [cathepsin B (CATB), cathepsin L (CATL)], the serine protease urokinase-type plasminogen activator (UPA) and its inhibitor type-1 (PAI-1) play an important part in cancer invasion. No data are available on the relationship between these proteases and gastric precancerous changes, Aims: To determine CATB, CATL, UPA, PAI-1 in chronic atrophic gastritis, intestinal metaplasia and gastric epithelial dysplasia, as precancerous changes, and to compare these data with those obtained in gastric cancer, Patients: Endoscopic biopsies were obtained from 12 patients with gastric cancer (cancerous tissue), 33 patients with chronic atrophic gastritis (all with intestinal metaplasia and 12 with dysplasia) and from 47 control subjects, for a total of 92 patients, Methods: Antigen concentrations were measured using ELISA methods, Immunohistochemistry was performed using monoclonal anti-CATB and anti-PAI-1 antibodies, Results: CATB, CATL, UPA and PAI-1 were significantly higher in chronic atrophic gastritis than in controls (CATB: P < 0.001; CATL: P < 0.005; UPA: P < 0.000001; PAI-1: P < 0.005), The same was observed for cancer, CATB and UPA were significantly higher in chronic atrophic gastritis, with versus without dysplasia (P < 0.05), Dysplastic epithelia showed strong immunoreactivity to PAI-1 and CATB, Conclusions: Our study demonstrates that cathepsins, UPA and PAI-1 may have a role not only in the process of cancer invasion, but also in the progression of precancerous changes into cancer.
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页码:2581 / 2587
页数:7
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