Spontaneous and radiation-induced chromosomal breakage at interstitial telomeric sites

被引：86

作者：

Slijepcevic, P ^{[1
]}

Xiao, Y ^{[1
]}

Dominguez, I ^{[1
]}

Natarajan, AT ^{[1
]}

机构：

[1] LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,NL-2300 RA LEIDEN,NETHERLANDS

来源：

CHROMOSOMA | 1996年 / 104卷 / 08期

关键词：

D O I：

10.1007/BF00352299

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Chinese hamster genome contains a total of 18 cytologically detectable arrays of interstitial telomeric sequences. A combination of G-banding and two-colour fluorescence in situ hybridization revealed that 25 out of 27 (93%) breakpoints of spontaneously occurring terminal deletions in four immortalized Chinese hamster cell lines were located in chromosomal regions containing interstitial telomeric sequences. Each of the four immortalized Chinese hamster cell lines expressed telomerase. Radiation experiments revealed the sensitivity of interstitial telomeric sequences to radiation-induced chromosomal breakage in all telomerase-positive cell lines. However, radiation-induced chromosomal breakage at interstitial telomeric sites in non-transformed, primary Chinese hamster cells was almost non-existent. Telomerase activity in primary Chinese hamster cells was not detected. These results indirectly suggest that interstitial telomeric sites represent a favourable substrate for chromosomal healing.

引用

页码：596 / 604

页数：9

共 40 条

[1] CHROMOSOMAL RADIOSENSITIVITY AT INTRACHROMOSOMAL TELOMERIC SITES
ALVAREZ, L
EVANS, JW
WILKS, R
LUCAS, JN
BROWN, JM
GIACCIA, AJ
[J]. GENES CHROMOSOMES & CANCER, 1993, 8 (01) : 8 - 14
[2] ANALYSIS OF RESTRICTION ENZYME-INDUCED CHROMOSOME-ABERRATIONS IN THE INTERSTITIAL TELOMERIC REPEAT SEQUENCES OF CHO AND CHE CELLS BY FISH
BALAJEE, AS
OH, HJ
NATARAJAN, AT
[J]. MUTATION RESEARCH, 1994, 307 (01): : 307 - 313
[3] CONSTRUCTION OF CHINESE-HAMSTER CHROMOSOME-SPECIFIC DNA LIBRARIES AND THEIR USE IN THE ANALYSIS OF SPONTANEOUS CHROMOSOME REARRANGEMENTS IN DIFFERENT CELL-LINES
BALAJEE, AS
DOMINGUEZ, I
NATARAJAN, AT
[J]. CYTOGENETICS AND CELL GENETICS, 1995, 70 (1-2): : 95 - 101
[4] TELOMERE DIRECTED FRAGMENTATION OF MAMMALIAN CHROMOSOMES
BARNETT, MA
BUCKLE, VJ
EVANS, EP
PORTER, ACG
ROUT, D
SMITH, AG
BROWN, WRA
[J]. NUCLEIC ACIDS RESEARCH, 1993, 21 (01) : 27 - 36
[5] TELOMERIC AND NONTELOMERIC (TTAGCC)(N) SEQUENCES IN GENE AMPLIFICATION AND CHROMOSOME STABILITY
BERTONI, L
ATTOLINI, C
TESSERA, L
MUCCIOLO, E
GIULOTTO, E
[J]. GENOMICS, 1994, 24 (01) : 53 - 62
[6] TELOMERASES
BLACKBURN, EH
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 : 113 - 129
[7] FUNCTIONAL-CHARACTERIZATION AND DEVELOPMENTAL REGULATION OF MOUSE TELOMERASE RNA
BLASCO, MA
FUNK, W
VILLEPONTEAU, B
GREIDER, CW
[J]. SCIENCE, 1995, 269 (5228) : 1267 - 1270
[8] MURINE RADIATION MYELOID LEUKEMOGENESIS - RELATIONSHIP BETWEEN INTERSTITIAL TELOMERE-LIKE SEQUENCES AND CHROMOSOME-2 FRAGILE SITES
BOUFFLER, S
SILVER, A
PAPWORTH, D
COATES, J
COX, R
[J]. GENES CHROMOSOMES & CANCER, 1993, 6 (02) : 98 - 106
[9] ISOLATION OF A YEAST CENTROMERE AND CONSTRUCTION OF FUNCTIONAL SMALL CIRCULAR CHROMOSOMES
CLARKE, L
CARBON, J
[J]. NATURE, 1980, 287 (5782) : 504 - 509
[10] TELOMERE SHORTENING ASSOCIATED WITH CHROMOSOME INSTABILITY IS ARRESTED IN IMMORTAL CELLS WHICH EXPRESS TELOMERASE ACTIVITY
COUNTER, CM
AVILION, AA
LEFEUVRE, CE
STEWART, NG
GREIDER, CW
HARLEY, CB
BACCHETTI, S
[J]. EMBO JOURNAL, 1992, 11 (05) : 1921 - 1929

← 1 2 3 4 →