The role of the cytoskeleton in the viscoelastic properties of human articular chondrocytes

被引:184
作者
Trickey, WR
Vail, TP
Guilak, F
机构
[1] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Biomed Engn, Orthopaed Res Labs, Durham, NC 27710 USA
关键词
cell mechanics; osteoarthritis; mechanical properties; actin; tubulm; virnentin; micropipette aspiration; biomechanics;
D O I
10.1016/S0736-0266(03)00150-5
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Biomechanical factors are believed to play an important role in regulating the metabolic activity of chondrocytes in articular cartilage. Previous studies suggest that cytoskeletal proteins such as actin, vimentin, and tubulin influence cellular mechanical properties, and may therefore influence the mechanical interactions between the chondrocyte and the surrounding tissue matrix. In this study, we investigated the role of specific cytoskeletal components on the mechanical properties of individual chondrocytes isolated from normal or osteoarthritic hip articular cartilage. Chondrocytes were exposed to a range of concentrations of chemical agents that disrupt the primary cytoskeletal elements (cytochalasin D for F-actin microfilaments, acrylamide for vimentin intermediate filaments, and colchicine for microtubules). Chondrocyte mechanical properties were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. Chondrocyte stiffness (elastic modulus) was significantly increased with osteoarthritis. With increasing cytochalasin D treatment, chondrocyte stiffness decreased by up to 90% and apparent viscosity decreased by up to 80%. The effect of cytochalasin D was greater on normal chondrocytes than those isolated from osteoarthritic cartilage. Treatment with acrylamide also decreased the moduli and viscosity, but only at the highest concentration tested. No consistent changes in cell mechanical properties were observed with colchicine treatment. These findings suggest that microfilaments and possibly intermediate filaments provide the viscoelastic properties of the chondrocyte, and changes in the structure and properties of these cytoskeletal elements may reflect changes in the chondrocyte with osteoarthritis. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:131 / 139
页数:9
相关论文
共 52 条
[1]   CYTOSKELETAL DYNAMICS IN RABBIT SYNOVIAL FIBROBLASTS .1. EFFECTS OF ACRYLAMIDE ON INTERMEDIATE FILAMENTS AND MICROFILAMENTS [J].
AGGELER, J ;
SEELY, K .
CELL MOTILITY AND THE CYTOSKELETON, 1990, 16 (02) :110-120
[2]  
[Anonymous], OSTEOARTHRITIS DIAGN
[3]   CYTOSKELETON OF CARTILAGE CELLS [J].
BENJAMIN, M ;
ARCHER, CW ;
RALPHS, JR .
MICROSCOPY RESEARCH AND TECHNIQUE, 1994, 28 (05) :372-377
[4]   ALTERATIONS IN CHONDROCYTE CYTOSKELETAL ARCHITECTURE DURING PHENOTYPIC MODULATION BY RETINOIC ACID AND DIHYDROCYTOCHALASIN-B INDUCED REEXPRESSION [J].
BROWN, PD ;
BENYA, PD .
JOURNAL OF CELL BIOLOGY, 1988, 106 (01) :171-179
[5]   EFFECT OF COLCHICINE ON VISCOELASTIC PROPERTIES OF NEUTROPHILS [J].
CHIEN, S ;
SUNG, KLP .
BIOPHYSICAL JOURNAL, 1984, 46 (03) :383-386
[6]  
DONG C, 1991, BIORHEOLOGY, V28, P557
[7]   Organisation of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture [J].
Durrant, LA ;
Archer, CW ;
Benjamin, M ;
Ralphs, JR .
JOURNAL OF ANATOMY, 1999, 194 :343-353
[8]  
ECKERT BS, 1985, EUR J CELL BIOL, V37, P169
[9]   APPARENT VISCOSITY AND CORTICAL TENSION OF BLOOD GRANULOCYTES DETERMINED BY MICROPIPET ASPIRATION [J].
EVANS, E ;
YEUNG, A .
BIOPHYSICAL JOURNAL, 1989, 56 (01) :151-160
[10]   Compression-induced changes in the shape and volume of the chondrocyte nucleus [J].
Guilak, F .
JOURNAL OF BIOMECHANICS, 1995, 28 (12) :1529-+