Evaluation of a Diagnostic Algorithm for Acute Q Fever in an Outbreak Setting

被引：22

作者：

Jager, Mischa M. ^{[1
,2
]}

Weers-Pothoff, Gezina ^{[1
]}

Hermans, Mirjam H. A.

Meekelenkamp, Jamie C. E. ^{[1
]}

Schellekens, Jeroen J. A.

Renders, Nicole H. M. ^{[1
]}

Leenders, Alexander C. A. P. ^{[1
]}

Schneeberger, Peter M. ^{[1
]}

Wever, Peter C. ^{[1
]}

机构：

[1] Jeroen Bosch Hosp, Dept Med Microbiol & Infect Control, NL-5200 ME Shertogenbosch, Netherlands

[2] Vrije Univ Amsterdam Med Ctr, Dept Med Microbiol & Infect Control, Amsterdam, Netherlands

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2011年 / 18卷 / 06期

关键词：

D O I：

10.1128/CVI.00009-11

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

In the peak of the 2009 Q fever outbreak in the Netherlands, we introduced a diagnostic algorithm for acute Q fever with an enzyme-linked immunosorbent assay for immunoglobulin M antibodies to Coxiella burnetii phase II antigens (MII screen) as an initial step. Subsequently, an immunofluorescence assay or PCR was performed depending on the MII screen outcome, date of onset of disease, and inpatient or outpatient setting. The impact of MII screen on the number of immunofluorescence assays performed and the contribution of PCR to diagnosis were retrospectively evaluated in 825 patients referred in a 17-day period. Acute Q fever was diagnosed in 256 patients. The introduction of MII screen reduced the number of immunofluorescence assays performed by more than 80%. In 103 patients, PCR analysis contributed to the diagnosis of acute Q fever. Q fever diagnostics were hampered by the fact that for a high number of patients the date of onset of disease was not provided and the requested follow-up serum samples were not received.

引用

页码：963 / 968

页数：6

共 7 条

[1]

[Anonymous], INFECTIEZIEKTEN B

[2]

Q fever. Was Edward Derrick's contribution undervalued? [J].