Phylogenetic relationships among Neofabraea species causing tree cankers and bull's-eye rot of apple based on DNA sequencing of ITS nuclear rDNA, mitochondrial rDNA, and the β-tubulin gene

被引:93
作者
de Jong, SN
Lévesque, CA
Verkley, GJM
Abeln, ECA
Rahe, JE
Braun, PG
机构
[1] Agr & Agri Food Canada, Pacific Agrifood Res Ctr, Summerland, BC V0H 1Z0, Canada
[2] Cent Bur Schimmelcultures, NL-3508 AD Utrecht, Netherlands
[3] Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada
[4] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, Kentville, NS B4N 1J5, Canada
来源
MYCOLOGICAL RESEARCH | 2001年 / 105卷
关键词
D O I
10.1017/S0953756201003926
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Three fungal species responsible for anthracnose canker, perennial canker, and bull's-eye rot of apple have been considered members of the genus Pezicula for a number of years. Recent studies, however, have provided evidence to (re-)classify these species as Neofabraea. There has been a long historical debate regarding the taxonomy of two of these fungi. In Europe, both Neofabraea malicorticis and N. perennans have generally been considered N. malicorticis, while in North America a species distinction has been maintained. Phylogenetic analyses of Neofabraea isolates were based on DNA sequences of the internal transcribed spacer region of nuclear rDNA (38 isolates), the mitochondrial rDNA small subunit (partial: 48 isolates), the beta -tubulin gene (partial: 25 isolates), and a combined data set (21 isolates). Our work provides evidence for the existence of four distinct Neofabraea apple pathogens including N. malicorticis, N. perennans, N. alba, and a putative new Neofabraea species that was isolated in both Europe and eastern North America. Our results indicate that the primary Neofabraea species causing tree cankers and bull's-eye rot in North America are N. malicorticis and N. perennans in the west and N. alba in eastern Canada. N. perennans. N. alba, and the undescribed Neofabraea species were found in Europe but the presence of N. malicorticis was not confirmed by our limited sampling. inclusion of Rosa spp. in the host range of N. malicorticis is merited.
引用
收藏
页码:658 / 669
页数:12
相关论文
共 37 条
[1]   Phylogeny of Pezicula, Dermea and Neofabraea inferred from partial sequences of the nuclear ribosomal RNA gene cluster [J].
Abeln, ECA ;
de Pagter, MA ;
Verkley, GJM .
MYCOLOGIA, 2000, 92 (04) :685-693
[2]   Comparison of AFLP fingerprints and ITS sequences as phylogenetic markers in Ustilaginomycetes [J].
Bakkeren, G ;
Kronstad, JW ;
Lévesque, CA .
MYCOLOGIA, 2000, 92 (03) :510-521
[3]  
BOEREMA G. H., 1959, TIJDSCHR PLANTEZIEKTEN, V65, P165, DOI 10.1007/BF01987776
[4]  
BOLAY A., 1956, TIJDSCHR PLANTENZIEKTEN, V62, P322, DOI 10.1007/BF01998531
[5]  
BOMPEIX G, 1974, Annales de Phytopathologie, V6, P1
[6]  
BOMPEIX G, 1974, Annales de Phytopathologie, V6, P13
[7]   DETECTION OF THE SOURCE OF INFECTION OF APPLE-TREES BY CYLINDROCARPON HETERONEMA USING DNA POLYMORPHISMS [J].
BROWN, AE ;
MUTHUMEENAKSHI, S ;
SWINBURNE, TR ;
LI, R .
PLANT PATHOLOGY, 1994, 43 (02) :338-343
[8]  
Childs L, 1927, ANN REPORT OREGON HO, V19, P108
[9]  
CREELMAN DW, 1962, CANADIAN PLANT DIS S, V42, P72
[10]  
DEJONG SN, 1998, THESIS S FRASER U BU