Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope

被引:301
作者
Heinz, WF [1 ]
Hoh, JH [1 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USA
关键词
D O I
10.1016/S0167-7799(99)01304-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The spatial distribution of intermolecular forces governs macromolecular interactions, The atomic force microscope, a relatively new tool for investigating interaction forces between nanometer-scale objects, can be used to produce spatially resolved maps of the surface or material properties of a sample; these include charge density, adhesion and stiffness, as well as the force required to break specific ligand-receptor bonds. Maps such as these will provide fundamental insights into biological structure and will become an important tool for characterizing technologically important biological systems.
引用
收藏
页码:143 / 150
页数:8
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