Tert-butyl hydroperoxide selectively inhibits mitochondrial respiratory-chain enzymes in isolated rat hepatocytes

被引:67
作者
Drahota, Z [1 ]
Kriváková, P
Cervinková, Z
Kmonícková, E
Lotková, H
Kucera, O
Houstek, J
机构
[1] Acad Sci Czech Republ, Inst Physiol, Videnska 1083, CR-14220 Prague, Czech Republic
[2] Acad Sci Czech Republ, Ctr Integrated Gen, CR-14220 Prague, Czech Republic
[3] Charles Univ Prague, Fac Med, Dept Physiol, Hradec Kralove, Czech Republic
[4] Acad Sci Czech Republ, Inst Expt Med, CR-14220 Prague, Czech Republic
关键词
hepatocytes; mitochondrial enzymes; tert-butyl hydroperoxide;
D O I
10.33549/physiolres.930578
中图分类号
Q4 [生理学];
学科分类号
071003 [生理学];
摘要
Sensitivity of various mitochondrial enzymes to oxidative damage was tested on isolated rat liver hepatocytes permeabilized. by digitonin. In permeabilized hepatocytes normal respiratory control values were obtained and mitochondrial membranes remained intact. Respiratory rates of NADH-dependent (glutamate+malate, palmitylcarnitine + malate) and flavoprotein-dependent (succinate) substrates were determined in hepatocytes exposed for 5 min to 0.5-3 mM tert-butyl hydroperoxide before addition of digitonin. Our data showed that oxidation of NADH-dependent substrates is much more sensitive to oxidative stress than oxidation of flavoprotein-dependent ones, evidently due to the modification of iron-sulfur clusters or SH groups in the NADH dehydrogenase enzyme complex (Complex I).
引用
收藏
页码:67 / 72
页数:6
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