Diagnostic potential of toscana virus N protein expressed in Escherichia coli

被引：18

作者：

Valassina, M

Soldateschi, D

Dal Maso, GM

Santini, L

Bianchi, S

Valensin, PE

Cusi, MG ^{[1
]}

机构：

[1] Univ Siena, Dept Mol Biol, Microbiol Sect, I-53100 Siena, Italy

[2] DIESSE Srl, I-53035 Monteriggioni, SI, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1998年 / 36卷 / 11期

关键词：

D O I：

10.1128/JCM.36.11.3170-3172.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA), The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.

引用

页码：3170 / 3172

页数：3

共 26 条

[1] TOSCANA VIRUS GENOMIC L-SEGMENT - MOLECULAR-CLONING, CODING STRATEGY AND AMINO-ACID-SEQUENCE IN COMPARISON WITH OTHER NEGATIVE STRAND RNA-VIRUSES [J].