Design and application of a peptide nucleic acid sequence targeting the p75 neurotrophin receptor

被引：4

作者：

Cheah, IK

Cheema, SS ^{[1
]}

Langford, SJ

Lopes, EC

Macfarlane, KJ

Petratos, S

Turner, BJ

机构：

[1] Monash Univ, Dept Anat & Cell Biol, Neurodegenerat Res Lab, Clayton, Vic 3800, Australia

[2] Monash Univ, Sch Chem, Clayton, Vic 3800, Australia

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2003年 / 13卷 / 14期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/S0960-894X(03)00400-1

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Novel antisense peptide nucleic acid (PNA) constructs targeting p75NTR as a potential therapeutic strategy for amyotrophic lateral sclerosis (ALS) were designed, synthesised and evaluated against phosphorothioate oligonucleotide sequences (PS-ODN). An 11-mer antisense PNA directed at the initiation codon dose-dependently inhibited p75NTR expression and death signalling by nerve growth factor in Schwann cell cultures. Inhibition of p75NTR production was not detected in cultures treated with the nonsense PNA or antisense PNA directed at the 3'-terminus sequence. The 19-mer PS-ODN sequences also failed to confer any activity against p75NTR but, unlike the PNA sequences, were toxic in vitro at comparable doses. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2377 / 2380

页数：4

共 21 条

[1] Dimethylcarbene, its radical cation and dication
Aubry, C
Polce, MJ
Holmes, JL
Mayer, PM
Radom, L
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (38) : 9039 - 9041
[2] Novel mechanisms for antisense-mediated regulation of gene expression
Baker, BF
Monia, BP
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1999, 1489 (01): : 3 - 18
[3] THE P75 NERVE GROWTH-FACTOR RECEPTOR MEDIATES SURVIVAL OR DEATH DEPENDING ON THE STAGE OF SENSORY NEURON DEVELOPMENT
BARRETT, GL
BARTLETT, PF
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (14) : 6501 - 6505
[4] Boado RJ, 2000, J PHARMACOL EXP THER, V295, P239
[5] Novel antisense and peptide nucleic acid strategies for controlling gene expression
Braasch, DA
Corey, DR
[J]. BIOCHEMISTRY, 2002, 41 (14) : 4503 - 4510
[6] Cheema SS, 1996, J NEUROSCI RES, V46, P239, DOI 10.1002/(SICI)1097-4547(19961015)46:2<239::AID-JNR12>3.0.CO
[7] 2-Y
[8] The neurotrophin receptor p75NTR:: novel functions and implications for diseases of the nervous system
Dechant, G
Barde, YA
[J]. NATURE NEUROSCIENCE, 2002, 5 (11) : 1131 - 1136
[9] Up-regulated p75NTR neurotrophin receptor on glial cells in MS plaques
Dowling, P
Ming, X
Raval, S
Husar, W
Casaccia-Bonnefil, P
Chao, M
Cook, S
Blumberg, B
[J]. NEUROLOGY, 1999, 53 (08) : 1676 - 1682
[10] Solution structure of a peptide nucleic acid DNA duplex
Eriksson, M
Nielsen, PE
[J]. NATURE STRUCTURAL BIOLOGY, 1996, 3 (05): : 410 - 413

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