A gene coding for tomato fruit β-galactosidase II is expressed during fruit ripening -: Cloning, characterization, and expression pattern

beta-Galactosidases (EC 3.2.1.23) constitute a widespread family of enzymes characterized by their ability to hydrolyze terminal, non-reducing beta-D-galactosyl residues from beta-D-galactosides, Several beta-galactosidases, sometimes referred to as exo-galactanases, have been purified from plants and shown to possess in vitro activity against extracted cell wall material via the release of galactose from wall polymers containing beta(1-->4)-D-galactan. Although p-galactosidase II, a protein present in tomato (Lycopersicon esculentum Mill.) fruit during ripening and capable of degrading tomato fruit galactan, has been purified, cloning of the corresponding gene has been elusive. We report here the cloning of a cDNA, pTom beta gal 4 (accession no. AF020390), corresponding to beta-galactosidase II, and show that its corresponding gene is expressed during fruit ripening. Northern-blot analysis revealed that the beta-galactosidase II gene transcript was detectable at the breaker stage of ripeness, maximum at the turning stage, and present at decreasing levels during the later stages of normal tomato fruit ripening. At the turning stage of ripeness, the transcript was present in all fruit tissues and was highest in the outermost tissues (including the peel). Confirmation that pTom beta gal 4 codes for beta-galactosidase II was derived from matching protein and deduced amino acid sequences. Furthermore, analysis of the deduced amino acid sequence of pTom beta gal 4 suggested a high probability for secretion based on the presence of a hydrophobic leader sequence, a leader-sequence cleavage site, and three possible N-glycosylation sites. The predicted molecular mass and isoelectric point of the pTom#gal 4-encoded mature protein were similar to those reported for the purified beta-galactosidase II protein from tomato fruit.