Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus)

被引：35

作者：

Mishra, N. ^{[1
]}

Dubey, R. ^{[1
]}

Rajukumar, K. ^{[1
]}

Tosh, C. ^{[1
]}

Tiwari, A. ^{[1
]}

Pitale, S. S. ^{[1
]}

Pradhan, H. K. ^{[1
]}

机构：

[1] Indian Vet Res Inst, High Secur Anim Dis Lab, Bhopal 462021, India

来源：

VETERINARY MICROBIOLOGY | 2007年 / 124卷 / 3-4期

关键词：

Bovine viral diarrhea virus (BVDV) type 2; goat; nested RT-PCR; phylogenetic analysis;

D O I：

10.1016/j.vetmic.2007.04.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent studies have shown that bovine viral diarrhea virus (BVDV) type I is widely prevalent in Indian cattle. In a surveillance of randomly collected 562 blood samples from seven states during 2004-2006, BVDV type 2 was detected in two native Indian goats by nested reverse transcription polymerase chain reaction (nRT-PCR). The virus isolated from them was classified antigenically as BVDV 2 on the basis of virus neutralization test and reactivity with monoclonal antibodies. Phylogenetic analysis of three different genomic regions, 5' un-translated region (5' UTR), E-rns structural coding region and NS5B nonstructural. coding region typed Indian goat isolate as BVDV 2a having close similarity with strains from North America and Europe suggesting its probable introduction through trade. It was placed in a separate clade within the 2a branch having unique mutations in E's and NS5B region. This is the first report of BVDV 2 in India and only second time recorded in goat species. The isolation of BVDV 2 from goat warrants intensive surveillance in cattle and sheep. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：340 / 347

页数：8

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