Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

被引：90

作者：

Shiku, H ^{[1
]}

Matsue, T ^{[1
]}

Uchida, I ^{[1
]}

机构：

[1] TOHOKU UNIV,GRAD SCH ENGN,DEPT APPL CHEM,SENDAI,MIYAGI 98077,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1996年 / 68卷 / 07期

关键词：

D O I：

10.1021/ac950824c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as similar to 10(4) CEA molecules in a single 20-mu m-radius spot.

引用

页码：1276 / 1278

页数：3

共 17 条

[1] ENZYME IMMUNOSENSOR .3. AMPEROMETRIC DETERMINATION OF HUMAN CHORIONIC-GONADOTROPIN BY MEMBRANE-BOUND ANTIBODY
AIZAWA, M
MORIOKA, A
SUZUKI, S
NAGAMURA, Y
[J]. ANALYTICAL BIOCHEMISTRY, 1979, 94 (01) : 22 - 28
[2] ADVANCES IN SCANNING ELECTROCHEMICAL MICROSCOPY - PLENARY LECTURE
ARCA, M
BARD, AJ
HORROCKS, BR
RICHARDS, TC
TREICHEL, DA
[J]. ANALYST, 1994, 119 (05) : 719 - 726
[3] CHEMICAL IMAGING OF SURFACES WITH THE SCANNING ELECTROCHEMICAL MICROSCOPE
BARD, AJ
FAN, FRF
PIERCE, DT
UNWIN, PR
WIPF, DO
ZHOU, FM
[J]. SCIENCE, 1991, 254 (5028) : 68 - 74
[4] SEPARATION-FREE SANDWICH ENZYME IMMUNOASSAYS USING MICROPOROUS GOLD ELECTRODES AND SELF-ASSEMBLED MONOLAYER IMMOBILIZED CAPTURE ANTIBODIES
DUAN, CM
MEYERHOFF, ME
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (09) : 1369 - 1377
[5] SCANNING ELECTROCHEMICAL MICROSCOPY
ENGSTROM, RC
PHARR, CM
[J]. ANALYTICAL CHEMISTRY, 1989, 61 (19) : A1099 - +
[6] ELECTROCHEMICAL DETECTION OF SINGLE MOLECULES
FAN, FRF
BARD, AJ
[J]. SCIENCE, 1995, 267 (5199) : 871 - 874
[7] GOSLING JP, 1990, CLIN CHEM, V36, P1408
[8] HAGE DS, 1995, ANAL CHEM, V67, pR455
[9] AUTOMATIC-APPARATUS FOR HETEROGENEOUS ENZYME IMMUNOASSAYS BASED ON ELECTROCATALYTIC DETECTION OF THE ENZYME AND ELECTROCHEMICAL REGENERATION OF THE SOLID-PHASE
HUET, D
BOURDILLON, C
[J]. ANALYTICA CHIMICA ACTA, 1993, 272 (02) : 205 - 212
[10] EXTENDING THE DETECTION LIMIT OF SOLID-PHASE ELECTROCHEMICAL ENZYME-IMMUNOASSAY TO THE ATTOMOLE LEVEL
JENKINS, SH
HEINEMAN, WR
HALSALL, HB
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 168 (02) : 292 - 299

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