Microfluidic gradient formation for nanoflow chip LC

被引：33

作者：

Brennen, Reid A. ^{[1
]}

Yin, Hongfeng ^{[1
]}

Killeen, Kevin P. ^{[1
]}

机构：

[1] Agilent Labs, Santa Clara, CA 95051 USA

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 24期

关键词：

D O I：

10.1021/ac0712805

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a method for forming a nanoflow liquid chromatography (nano-LC) gradient using a single fluid pump at flow rates below 1 mu L/min by passively forming a gradient on a microfluidic device. This device works together with an Agilent HPLC-Chip to perform high-throughput nanoflow liquid chromatography/mass spectrometry (nano-LC/MS)The nanoflow gradient delay time is reduced from several minutes for a commercial LC nanoflow pump to only a few seconds with this microfluidic device, thus shortening the total analysis time and increasing the analysis throughput. With this microfluidic device, a nano-LC solvent delivery system can be greatly simplified and have increased robustness, reliability, reduced waste, and ease of use.

引用

收藏

页码：9302 / 9309

页数：8

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