A RAPD-PCR method for large-scale typing of Bacillus cereus

被引：32

作者：

Nilsson, J ^{[1
]}

Svensson, B ^{[1
]}

Ekelund, K ^{[1
]}

Christiansson, A ^{[1
]}

机构：

[1] Swedish Dairies Assoc, Dept Res & Dev, S-22370 Lund, Sweden

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1998年 / 27卷 / 03期

关键词：

D O I：

10.1046/j.1472-765X.1998.00402.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A robust RAPD-PCR procedure for large-scale typing of Bacillus cereus was developed. It is based on a simple DNA preparation, involving only freezing and boiling of cells in water with active carbon. By using a computerized system for data collection and processing, an efficient system for handling RAPD patterns for large-scale investigations was achieved. The procedure sr as highly discriminatory for Bacillus cereus strains anti was found to give reproducible classification of RAPD fingerprints for fire reference strains.

引用

页码：168 / 172

页数：5

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