Saccharomyces cerevisiae mitochondria can synthesise FMN and FAD from externally added riboflavin and export them to the extramitochondrial phase

被引：52

作者：

Pallotta, ML

Brizio, C

Fratianni, A

De Virgilio, C

Barile, M

Passarella, S

机构：

[1] Univ Molise, Dipartimento Sci Anim Vegetali & Ambiente, I-86100 Campobasso, Italy

[2] Univ Bari, Dipartmento Biochim & Biol Mol, Bari, Italy

来源：

FEBS LETTERS | 1998年 / 428卷 / 03期

关键词：

mitochondrion; riboflavin; flavin adenine dinucleotide; flavin mononucleotide; Saccharomyces cerevisiae;

D O I：

10.1016/S0014-5793(98)00544-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Evidence is given that mitochondria isolated from Saccharomyces cerevisiae can take up externally added riboflavin and synthesise from it both flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) probably due to the existence of the mitochondrial riboflavin kinase already reported and the novel mitochondria FAD synthetase. Moreover Saccharomyces cerevisiae mitochondria can export the newly synthesised flavin derivatives to the extramitochondrial phase. This has been proven to take place with 1:1 stoichiometry with riboflavin decrease outside mitochondria, thus showing that flavin traffic occurs across the mitochondrial membranes. (C) 1998 Federation of European Biochemical Societies.

引用

页码：245 / 249

页数：5

共 14 条

[1]

BACHER A, 1990, CHEM BIOCH FLAVOENZY, P349

[2] Flavin adenine dinucleotide and flavin mononucleotide metabolism in rat liver - The occurrence of FAD pyrophosphatase and FMN phosphohydrolase in isolated mitochondria [J].