Analysis of sugar phosphates and related compounds using capillary zone electrophoresis with indirect UV detection

Sugar phosphates and related compounds can be rapidly separated using capillary zone electrophoresis and quantitated via indirect UV detection using potassium sorbate at 256 nm. Three buffers were examined at various pH values. The optimum separation conditions resulted with 6 mM potassium sorbate at pH 5.8. Pairs of regioisomers such as glucose-l-phosphate and glucose-6-phosphate or ribose-l-phosphate and ribose-5-phosphate were readily separated. A mixture of seven sugar phosphates and orthophosphate also was separated. The detection limits for samples of glucose-6-phosphate and glucose-1-phosphate were 0.14 mM and 0.26 mM, respectively. (C) 1998 Elsevier Science B.V. All rights reserved.